| Citation: |
Lü ZH，Han KN，Du XF，Wang ZL，Li YX，Lian SC，Zhang LY，Wang J. Identification and application of salt-tolerant germplasms at germination stage in foxtail millet(Setaria italica L.)[J]. Plant Science Journal，2024，42（1）：75−84. DOI: 10.11913/PSJ.2095-0837.23055
|
In this study, 10 foxtail millet (Setaria italica L.) germplasm accessions were treated with varying concentrations of NaCl solution. By analyzing four indexes, including the relative germination potential, relative germination rate, relative shoot length, and relative root length during germination stage, we established that the suitable NaCl concentration for the identification of salt tolerance during germination in foxtail millet was 180 mmol/L. To evaluate the salt-tolerance of 180 core germplasms during the germination stage, correlation analysis and principal component analysis were employed to calculate the aforementioned four traits under 180 mmol/L NaCl treatment. Comprehensive evaluation and salt-tolerant grade of the 180 germplasms were identified by membership functional analysis and cluster analysis. Results revealed that except for the non-significant correlation between relative germination rate and relative shoot length, all other indexes showed a highly significant positive correlation. Furthermore, principal component analysis suggested that the four indexes could be considered as key indices for assessing salt tolerance. Cluster analysis revealed that the 180 germplasms could be divided into four groups: highly salt tolerant, salt tolerant, salt sensitive, and extremely salt sensitive. Jiangu, Henggu 12, Qitoubai, K-3606, and Jingu 20 were identified as extremely salt tolerant accessions through comprehensive evaluation using membership function. In addition, under 180 mmol/L NaCl stress, 40 lines from the F7 generation recombinant inbred line (RIL) of Heizhigu (extremely salt-sensitive)×Changnong 35 (salt-tolerant) were analyzed. Results showed significant variation among the 40 lines, with their frequency distribution of salt tolerance grades approximating normal distribution, indicating that this population was suitable for salt-tolerant QTL mapping. These findings demonstrated that 180 mmol/L NaCl could be used for the identification of salt-tolerant foxtail millet germplasms during the germination stage and that at this concentration, salt tolerance differences among various germplasms can be better distinguished by four indexes: relative germination potential, relative germination rate, relative shoot length, and relative root length.
| [1] |
王遵亲. 中国盐渍土[M]. 北京: 科学出版社, 1993: 400-515.
|
| [2] |
中国科学院国家计划委员会, 自然资源考察委员会. 《中国1∶100万土地资源图》土地资源数据集[M]. 北京: 中国人民大学出版社, 1991: 89-175.
|
| [3] |
全国土壤普查办公室. 中国土壤[M]. 北京: 中国农业出版社, 1998: 1253.
|
| [4] |
马子林,马玉兰. 盐碱地的形成原因及改良措施[J]. 青海农牧业,2007(2):18.
|
| [5] |
黄琼. 浅谈盐碱地的改良措施与对策[J]. 现代园艺,2016(22):230−231.
|
| [6] |
殷炳政,张怀东,周彬. 农业综合开发治理盐碱地措施和方法初探[J]. 山东省农业管理干部学院学报,2013,30(4):38−39.
|
| [7] |
袁杰,王学强,张燕红,赵志强,贾春平,等. 水稻种质资源苗期耐盐性鉴定[J]. 分子植物育种,2020,18(20):6808−6814.
Yuan J,Wang XQ,Zhang YH,Zhao ZQ,Jia CP,et al. Identification and screening of salt tolerance in rice germplasm resources at seedling stage[J]. Molecular Plant Breeding,2020,18(20):6808−6814.
|
| [8] |
李小康,吴崇宁,王维,李文淑,Masahiro K,等. 人工合成六倍体小麦耐盐种质资源的筛选及评价[J]. 麦类作物学报,2021,41(12):1487−1495.
Li XK,Wu CN,Wang W,Li WS,Masahiro K,et al. Screening and evaluation of salt-tolerant germplasm of synthetic hexaploidy wheat[J]. Journal of Triticeae Crops,2021,41(12):1487−1495.
|
| [9] |
段雅娟,曹士亮,于滔,李文跃,杨耿斌,等. 玉米自交系萌发期耐盐性鉴定[J]. 作物杂志,2022(1):213−219.
Duan YJ,Cao SL,Yu T,Li WY,Yang GB,et al. Identification of salt tolerance during germination of maize inbred lines[J]. Crops,2022(1):213−219.
|
| [10] |
胡亮亮,王素华,王丽侠,程须珍,陈红霖. 绿豆种质资源苗期耐盐性鉴定及耐盐种质筛选[J]. 作物学报,2022,48(2):367−379. doi: 10.3724/SP.J.1006.2022.04283
Hu LL,Wang SH,Wang LX,Cheng XZ,Chen HL. Identification of salt tolerance and screening of salt tolerant germplasm of mungbean (Vigna radiate L. ) at seedling stage[J]. Acta Agronomica Sinica,2022,48(2):367−379. doi: 10.3724/SP.J.1006.2022.04283
|
| [11] |
张海金. 谷子在旱作农业中的地位和作用[J]. 安徽农学通报,2007,13(10):169−170.
|
| [12] |
张一中,张一弓,柳青山. 谷子在山西省旱作农业中的地位和作用[J]. 中国种业,2011(8):21−22.
|
| [13] |
刁现民. 中国谷子生产与发展方向[M]//柴岩, 万福世, 编. 中国小杂粮产业发展报告. 北京: 中国农业出版社, 2007: 32-43.
|
| [14] |
韩飞,诸葛玉平,娄燕宏,王会,张乃丹,等. 63份谷子种质的耐盐综合评价及耐盐品种筛选[J]. 植物遗传资源学报,2018,19(4):685−693.
Han F,Zhuge YP,Lou YH,Wang H,Zhang ND,et al. Evaluation of salt tolerance and screening for salt tolerant accessions of 63 foxtail millet germplasm[J]. Journal of Plant Genetic Resources,2018,19(4):685−693.
|
| [15] |
秦岭,张艳亭,陈二影,杨延兵,黎飞飞,管延安. 谷子萌发期耐盐种质筛选及其保护酶系统对盐胁迫的响应[J]. 中国农业科学,2019,52(22):4027−4038.
Qin L,Zhang YT,Chen EY,Yang YB,Li FF,Guan YA. Screening for germplasms tolerant to salt at germination stage and response of protective enzymes to salt stress in foxtail millet[J]. Scientia Agricultura Sinica,2019,52(22):4027−4038.
|
| [16] |
张笛. 谷子耐盐种质资源筛选及生长发育与农艺性状的鉴定[D]. 黑龙江: 黑龙江八一农垦大学, 2020: 5-60.
|
| [17] |
田伯红,王素英,李雅静,王建广,张立新,等. 谷子地方品种发芽期和苗期对NaCl胁迫的反应和耐盐品种筛选[J]. 作物学报,2008,34(12):2218−2222.
Tian BH,Wang SY,Li YJ,Wang JG,Zhang LX,et al. Response to sodium chloride stress at germination and seedling and identification of salinity tolerant genotypes in foxtail millet landraces originated from China[J]. Acta Agronomica Sinica,2008,34(12):2218−2222.
|
| [18] |
张笛,苗兴芬,王雨婷. 100份谷子品种资源萌发期耐盐性评价及耐盐品种筛选[J]. 作物杂志,2019(6):43−49.
Zhang D,Miao XF,Wang YT. Evaluation and screening of salt tolerance in 100 foxtail millet at germination stage[J]. Crops,2019(6):43−49.
|
| [19] |
谢志坚. 农业科学中的模糊数学方法[M]. 武汉: 华中理工大学出版社, 1983: 99-193.
|
| [20] |
戴海芳,武辉,阿曼古丽·买买提阿力,王立红,麦麦提·阿皮孜,等. 不同基因型棉花苗期耐盐性分析及其鉴定指标筛选[J]. 中国农业科学,2014,47(7):1290−1300.
Dai HF,Wu H,Amanguli · Maimaitiali,Wang LH,Maimaiti · Apizi,et al. Analysis of salt-tolerance and determination of salt-tolerant evaluation indicators in cotton seedlings of different genotypes[J]. Scientia Agricultura Sinica,2014,47(7):1290−1300.
|
| [21] |
Khan AA,Rao SA,McNeilly T. Assessment of salinity tolerance based upon seedling root growth response functions in maize (Zea mays L.)[J]. Euphytica,2003,131(1):81−89. doi: 10.1023/A:1023054706489
|
| [22] |
Verma OPS,Yadava RBR. Salt tolerance of some oats (Avena sativa L.) varieties at germination and seedling stage[J]. J Agron Crop Sci,1986,156(2):123−127. doi: 10.1111/j.1439-037X.1986.tb00016.x
|
| [23] |
智慧,刁现民,吕芃,李伟,Akolavo Z. 人工盐胁迫法鉴定谷子及狗尾草物种耐盐基因型[J]. 河北农业科学,2004,8(4):15−18.
Zhi H,Diao XM,Lü P,Li W,Akolavo Z. Methodology analysis on screening of salt tolerant genotypes from foxtail millet and other Setaria species[J]. Journal of Hebei Agricultural Sciences,2004,8(4):15−18.
|
| [24] |
崔兴国,时丽冉. 衡水地区14份夏谷品种种子萌发期耐盐性研究[J]. 作物杂志,2011(4):117−119.
Cui XG,Shi LR. Evaluation of salt tolerance of 14 foxtail millet summer varieties in Hengshui[J]. Crops,2011(4):117−119.
|
| [25] |
田伯红,王建广,李雅静,张立新,孔德平. 谷子发芽期和幼苗前期耐盐性鉴定指标的研究[J]. 河北农业科学,2008,12(7):4−6.
Tian BH,Wang JG,Li YJ,Zhang LX,Kong DP. Studies on screening indexes for salt tolerance of millet during germination period and early seedling stage[J]. Journal of Hebei Agricultural Sciences,2008,12(7):4−6.
|
| [26] |
Ardie SW,Khumaida N,Nur A,Fauziah N. Early identification of salt tolerant foxtail millet (Setaria italica L. Beauv)[J]. Proc Food Sci,2015,3:303−312. doi: 10.1016/j.profoo.2015.01.033
|
| [27] |
Pan JW,Li Z,Dai SJ,Ding HF,Wang QG,et al. Integrative analyses of transcriptomics and metabolomics upon seed germination of foxtail millet in response to salinity[J]. Scientific Reports,2020,10(1):13660. doi: 10.1038/s41598-020-70520-1
|
| [28] |
陈二影,秦岭,杨延兵,黎飞飞,王润丰,等. 生产条件下谷子品种盐碱耐性的差异及综合评价[J]. 中国农业科学,2019,52(22):4050−4065.
Chen EY,Qin L,Yang YB,Li FF,Wang RF,et al. Variation and comprehensive evaluation of salt and alkali tolerance of different foxtail millet cultivars under production conditions[J]. Scientia Agricultura Sinica,2019,52(22):4050−4065.
|
| [29] |
张艳亭. 谷子种质资源耐盐性鉴定和耐盐机理的研究[D]. 曲阜: 曲阜师范大学, 2018: 7-9.
|
| [30] |
郭瑞锋,张永福,任月梅,杨忠. 混合盐碱胁迫对谷子萌发、幼芽生长的影响及耐盐碱品种筛选[J]. 作物杂志,2017(4):63−66.
Guo RF,Zhang YF,Ren YM,Yang Z. Effects of saline-alkali stress on millet germination and shoots growth and saline-alkali tolerance variety screening[J]. Crops,2017(4):63−66.
|
| [31] |
陈二影,王润丰,秦岭,杨延兵,黎飞飞,等. 谷子芽期耐盐碱综合鉴定及评价[J]. 作物学报,2020,46(10):1591−1604.
Chen EY,Wang RF,Qin L,Yang YB,Li FF,et al. Comprehensive identification and evaluation of foxtail millet for saline-alkaline tolerance during germination[J]. Acta Agronomica Sinica,2020,46(10):1591−1604.
|
| [32] |
虞晓芬,傅玳. 多指标综合评价方法综述[J]. 统计与决策,2004(11):119−121.
|
| [33] |
张巧凤,陈宗金,吴纪中,蒋彦婕,杨继书,等. 小麦种质芽期和苗期的耐盐性鉴定评价[J]. 植物遗传资源学报,2013,14(4):620−626.
Zhang QF,Chen ZJ,Wu JZ,Jiang YJ,Yang JS,et al. Screening for salinity tolerance at germination and seedling stages in wheat germplasm[J]. Journal of Plant Genetic Resources,2013,14(4):620−626.
|
| [34] |
周和平,张立新,禹锋,李平. 我国盐碱地改良技术综述及展望[J]. 现代农业科技,2007(11):159−161.
|
| [35] |
Ren ZH,Gao JP,Li LG,Cai XL,Huang W,et al. A rice quantitative trait locus for salt tolerance encodes a sodium transporter[J]. Nat Genet,2005,37(10):1141−1146. doi: 10.1038/ng1643
|
| [36] |
Niones JM. Five mapping of the salinity tolerance gene on chromosome 1 of rice （Oryza sativa L.） using near-isogenic lines[M]. Laguna: University of the Philippines, 2004: 1-100.
|
| [1] | Chen Yao, Zhang Zhi-Peng, Zhang Zhao, Zhang Yang, Xie Cai-Xiang, Xu Shuo. Effect of soil factors on the content of chemical components in Phellodendri Amurensis Cortex[J]. Plant Science Journal, 2019, 37(6): 797-807. DOI: 10.11913/PSJ.2095-0837.2019.60797 |
| [2] | ZHANG Run-Hua, LI Zhi-Guo, LIU Xu-Dong, WANG Bin-Cai, WANG Ai-Hua, HUANG Xing-Xue, ZHOU Guo-Lin, QIAN Yun-Guo. Impact of Cultivation Change from Open Field to Greenhouse on Heavy Metal Contents and Fractions of Soil[J]. Plant Science Journal, 2016, 34(4): 575-582. DOI: 10.11913/PSJ.2095-0837.2016.40575 |
| [3] | LIU Hai-Yan, HUANG Cai-Mei, ZHOU Sheng-Yong, HUANG Li-Hua, ZOU Tian-Cai. Study on the Content Changes of Zinc and Selenium in Tea and Differences in the Planting Soil[J]. Plant Science Journal, 2015, 33(2): 237-243. DOI: 10.11913/PSJ.2095-0837.2015.20237 |
| [4] | OUYANG Zheng-Rong, WEN Xiao-Bin, GENG Ya-Hong, MEI Hong, HU Hong-Jun, ZHANG Gui-Yan, LI Ye-Guang. The Effects of Light Intensities, Temperatures, pH and Salinities on Photosynthesis of Chlorella[J]. Plant Science Journal, 2010, 28(1): 49-55. |
| [5] | ZHANG Yue-Jin, MENG Xiang-Hai, YANG Dong-Feng, XU Ling, ZHANG Xiao-Yan. Comparative Study on Chemical Constituents of Pinellia ternata(Thunb.) Breit.under Different Light Intensities[J]. Plant Science Journal, 2009, 27(5): 533-536. |
| [6] | MU Hong-Ping, YE Wan-Hui, CHEN Yi-Zhu, ZHU Jian-Ling, CAO Hong-Lin. Photosynthesis and Growth of Ardisia crenata and Ardisia punctata under Different Phosphorus Nutrition Levels[J]. Plant Science Journal, 2008, 26(5): 514-519. |
| [7] | CHEN Xiao-Feng, WANG Qing-Ya, CHEN Kai-Ning. Impacts of Different Light Intensity on Morphology and Structure of Potamogeton crispus[J]. Plant Science Journal, 2008, 26(2): 163-169. |
| [8] | XU Kai-Yang, YE Wan-Hui, LI Guo-Min, LI Jing. Phenotypic Plasticity in Response to Light Intensity in the Invasive Species Alternanthera philoxeroides[J]. Plant Science Journal, 2005, 23(6): 560-563. |
| [9] | LIU Bing-Qin, WANG Wan-Xian, SONG Chun-Lei, CAO Xiu-Yun, ZHOU Yi-Yong. Influences of Potamogeton crispus L.on Phosphorus Status in Lake Sediments[J]. Plant Science Journal, 2004, 22(5): 394-399. |
| [10] | Wang Fengchun, Liu Mingyuan. BIOSYSTEMATIES OF TRIFOLIUM PRATENSE L.IN THE EAST MOUNTAINS IN HEILONGJIANG PROVINCE, CHINA[J]. Plant Science Journal, 1989, 7(4): 317-326. |
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: