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LI Yan-Kun, TAO Ai-Fen, QI Jian-Min, ZHOU Dong-Xin, WANG Tao, ZHANG Guang-Qing, WEI Cheng-Lin, LAN Tao, CHEN Shun-Hui. Polymorphic Analysis of RAPD with Double Primers and a Single Primer in Tobacco[J]. Plant Science Journal, 2010, 28(3): 257-262. DOI: 10.3724/SP.J.1142.2010.30257
Citation: LI Yan-Kun, TAO Ai-Fen, QI Jian-Min, ZHOU Dong-Xin, WANG Tao, ZHANG Guang-Qing, WEI Cheng-Lin, LAN Tao, CHEN Shun-Hui. Polymorphic Analysis of RAPD with Double Primers and a Single Primer in Tobacco[J]. Plant Science Journal, 2010, 28(3): 257-262. DOI: 10.3724/SP.J.1142.2010.30257
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Polymorphic Analysis of RAPD with Double Primers and a Single Primer in Tobacco

  • 1.

    Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,Fujian Agriculture and ForestryUniversity, Fuzhou 350002,China

  • 2.

    Bureau of Agriculture of Qihe County of Shandong Province, Qihe,Shandong 251100,China

  • 3.

    3.Longyan Tobacco Branch Company, Longyan,Fujian 364000,China

  • 4.

    Nanping Tobacco Company, Nanping,Fujian 353200,China

  • Insititute of Crop Science,Academy of Agriculture Science of Fujian Province, Fuzhou 350003,China

  • 6.

    6.Fuzhou Institute of Tobacco Science of Fujian Province, Fuzhou 350003,China

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  • Received Date: December 31, 1899
  • Revised Date: December 31, 1899
  • Published Date: June 29, 2010
    Graphical Abstract
    • Abstract
  • Two materials with a relatively wide genetic relationship(GS=0.58),Taiyan 7 and Burley 21,were employed to study the effect of double-primers on the polymorphism of the RAPD PCR reaction,using the standard RAPD procedure(single primer) as a reference.Results showed that the double-primers reaction could amplify more polymorphic bands than the standard RAPD technique.The application of the double-primers RAPD technique could,therefore,be an effective and economical method to improve the selection efficiency of polymorphism molecular markers.
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