Cloning and Bioinformatics Analysis of IQM2 cDNA from Arabidopsis

Arabidospsis IQM2,the second member of the IQM family,is encoded by At3g13600. Its cDNA sequence,however,can not be found in any molecular biology literature or database.Using RACE and RT-PCR methods,a 2245 bp long full-length cDNA of Arabidopsis IQM2 was cloned in this study.Its open reading frame (ORF) was 1818 bp long and encoded for a 605-amino-acid polypeptide.Bioinformatics analysis revealed that the polypeptide had an IQ motif and shared sequence homology with the pea heavy metal induced protein 6A in its N-terminus and a ribosome inactivating protein,trichosanthin,in its C-terminus.The IQ motif located in the homologous region of the pea heavy metal -induced protein 6A.