Callus Induction and Plant Regeneration of Fagopyrum dibotrys (D.Don) Hara, An Important Medicinal Plant

Transgenic technology has now become one of the most important measures in plant directional genetic improvement,while the establishment of a high-efficiency regenerating system must be the basis and prerequisite of genetic transformation.In the present research,explants (leaf,petiole and stem segments) excised from 25-30 day-old in vitro seedlings of Fagopyrum dibotrys were used as materials for inducing callus and plant regeneration.Results showed that MS+2,4-D 4.0 mg/L+6-BA 1.0 mg/L was beneficial for leaves to form callus (89%).Internodal cuttings formed callus (87%) on MS medium supplemented with 2,4-D 2.0 mg/L+6-BA 1.0-2.0 mg/L,and the highest callus induction from the petiole was only 54% on MS+2,4-D 4.0 mg/L+6-BA 2.0 mg/L+IBA 0.2 mg/L media.The medium of MS+6-BA 2.0 mg/L+TDZ 0.2 mg/L+NAA 0.2 mg/L was prior to callus differentiation and shoot organogenesis.The optimum medium for producing roots (96.67%) was half-strength MS medium supplemented with NAA (0.5 mg/L).Hardened plantlets via acclimatization were successfully transplanted to a field (80%),with the regenerated plants normal morphologically and in growth characters.The high-efficient stable organogenesis regeneration system of Fagopyrum dibotrys will make it possible for genetic transformation and enlarging medical resources for this species.