Advance Search
WEI Jin-Chi, YANG Hai-Ling. Functional Divergence of Two Glutathione Peroxidase Genes in Oryza sativa[J]. Plant Science Journal, 2013, 31(1): 64-72. DOI: 10.3724/SP.J.1142.2013.10064
Citation: WEI Jin-Chi, YANG Hai-Ling. Functional Divergence of Two Glutathione Peroxidase Genes in Oryza sativa[J]. Plant Science Journal, 2013, 31(1): 64-72. DOI: 10.3724/SP.J.1142.2013.10064
shu

Functional Divergence of Two Glutathione Peroxidase Genes in Oryza sativa

  • 1.

    College of Life Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China

  • 2. Key Laboratory of Tree and Ornamental Plant Breeding and Biotechnology of State Forestry Administration, Beijing 100083, China

More Information
  • Received Date: June 12, 2012
  • Revised Date: October 20, 2012
  • Published Date: February 27, 2013
    Graphical Abstract
    • Abstract
  • Plant glutathione peroxidases (GPX) are a group of enzymes that regulate levels of reactive oxygen species in cells and tissues,and protect them against oxidative damage.In this study,two GPX genes (OsGPX3 and OsGPX4) were cloned from Oryza sativa.The OsGPX3 and OsGPX4 genes encoded two proteins of 238 and 234 amino acid residues,with calculated molecular masses of 25.84 kDa and 25.07 kDa,respectively.Genomic sequence analysis showed OsGPX3 and OsGPX4 contained five introns.Reverse transcription PCR revealed that OsGPX3 and OsGPX4 were constitutive expression genes in O.sativa.The OsGPX3 and OsGPX4 proteins were overexpressed in E.coli,and were purified by Ni-affinity chromatography.The OsGPX3 and OsGPX4 proteins showed enzymatic activities towards substrates H2O2,tBOOH and COOH.However,OsGPX3 showed higher enzymatic activities to the three substrates than did OsGPX4,suggesting functional divergence between the OsGPX3 and OsGPX4 genes.
    • FullText(HTML)
    • References (15)
  • [1]
    Apel K,Hirt H.Reactive oxygen species: metabolism,oxidative stress,and signal transduction[J].Ann Rev Plant Biol, 2004, 55: 373-399.
    [2]
    Navrot N,Collin V,Gualberto J,Gelhaye E,Hirasawa M,Rey P,Knaff D B,Issakidis E,Jacquot J P,Rouhier N.Plant glutathione peroxidases are functional peroxiredoxins distributed in several subcellular compartments and regulated during biotic and abiotic stresses[J].Plant Physiol, 2006, 142: 1364-1379.
    [3]
    Arthur J R.The glutathione peroxidases[J].Cell Mol Life Sci, 2000, 57: 1825-1835.
    [4]
    Miao Y,Lv D,Wang P,Wang X C,Chen J,Miao C,Song C P.An Arabidopsis glutathione peroxidase functions as both a redox transducer and a scavenger in abscisic acid and drought stress responses[J].Plant Cell, 2006, 18: 2749-2766.
    [5]
    Chang C C,Slesak I,Jorda L,Sotnikov A,Melzer M,Miszalski Z,Mullineaux P M,Parker J E,Karpinska B,Karpinski S.Arabidopsis chloroplastic glutathione peroxidases play a role in cross talk between photooxidative stress and immune responses[J].Plant Physiol, 2009, 150: 670-683.
    [6]
    Chen S,Vaghchhipawala Z,Li W,Asard H,Dickman M B.Tomato phospholipid hydroperoxide glutathione peroxidase inhibits cell death induced by Bax and oxidative stresses in yeast and plants[J].Plant Physiol, 2004, 135: 1630-1641.
    [7]
    Criqui M C,Jamet E,Parmentier Y,Marbach J,Durr A,Fleck J.Isolation and characterization of a plant cDNA showing homology to animal glutathione peroxidases[J].Plant Mol Biol, 1992, 18: 623-627.
    [8]
    Sugimoto M,Furui S,Suzuki Y.Molecular cloning and characterization of a cDNA encoding putative phospholipid hydroperoxide glutathione peroxidase from spinach[J].Biosci Biotechnol Biochem, 1997, 61: 1379-1381.
    [9]
    Wang Z,Wang F,Duan R,Liu J Y.Purification and physicochemical characterization of a recombinant phospholipid hydroperoxide glutathione peroxidase from Oryza sativa[J].J Biochem Mol Biol, 2007, 40: 412-418.
    [10]
    Margis R,Dunand C,Teixeira F K,Margis-Pinheiro M.Glutathione peroxidase family — an evolutionary overview[J].FEBS J, 2008, 275: 3959-3970.
    [11]
    Li W J,Feng H,Fan J H,Zhang R Q,Zhao N M,Liu J Y.Molecular cloning and expression of a phospholipid hydroperoxide glutathione peroxidase homolog in Oryza sativa[J].Biochim Biophys Acta, 2000, 1493: 225-230.
    [12]
    Kang S G,Jeong H K,Suh H S.Characterization of a new member of the glutathione peroxidase gene family in Oryza sativa[J].Mol Cells, 2004, 17: 23-28.
    [13]
    Yang X,Sun W,Liu J P,Liu Y J,Zeng Q Y.Biochemical and physiological characterization of a tau class glutathione transferase from rice (Oryza sativa)[J].Plant Physiol Biochem, 2009, 47: 1061-1068.
    [14]
    Jung B G,Lee KO,Lee S S,Chi Y H,Jang H H,Kang S S,Lee K,Lim D,Yoon S C,Yun D J,Inoue Y,Cho M J,Lee S Y.A Chinese cabbage cDNA with high sequence identity to phospholipid hydroperoxide glutathione peroxidases encodes a novel isoform of thioredoxin-dependent peroxidase[J].J Biol Chem, 2002, 277: 12572-12578.
    [15]
    苗雨晨,白玲,苗琛,陈珈,宋纯鹏.植物谷胱甘肽过氧化物酶研究进展[J].植物学通报, 2005, 22(3): 350-356.
    • Related Articles

  • Related Articles

    [1]Chen Xiao-Hong, Wei Li, Huang Yu-Qiong, Chen Li-Qing. Study on the physiological and biochemical characteristics of Brunfelsia acuminata petals during flowering[J]. Plant Science Journal, 2018, 36(4): 595-602. DOI: 10.11913/PSJ.2095-0837.2018.40595
    [2]YANG Zhong-Min, WANG Yan. Cloning of Potassium Transporter Gene (HcKUP12) from Halostachys caspica and Its Expression Profile under Salt Stress[J]. Plant Science Journal, 2015, 33(4): 499-506. DOI: 10.11913/PSJ.2095-0837.2015.40499
    [3]XIONG Bo, WANG Zhi-Hui, LIAO Feng-Ling, FAN Qian-Qian, LI Li-Jiao, WANG Xiao-Xiao. Effects of Calcium on the Activities of Three Antioxidant Enzymes in Huangguogan Fruit[J]. Plant Science Journal, 2014, 32(2): 168-173. DOI: 10.3724/SP.J.1142.2014.20168
    [4]WANG Yan, CHEN Xi, YANG Zhong-Min, LIU Deng-Hui. Cloning of Pathogen-Related Protein Gene(SfPR-1) from Salsola ferganica and Its Expression Analysis under Salt Stress[J]. Plant Science Journal, 2013, 31(2): 164-170. DOI: 10.3724/SP.J.1142.2013.20164
    [5]CHEN Tao, WANG Gui-Mei, SHEN Wei-Wei, LI Xiao-Zhen, QI Jian-Min, XU Jian-Tang, TAO Ai-Fen, LIU Xiao-Qian. Effect of Salt Stress on the Growth and Antioxidant Enzyme Activity of Kenaf Seedlings[J]. Plant Science Journal, 2011, 1(4): 493-501.
    [6]HU Xiao-Hui, GUO Shi-Rong, LI Jing, WANG Su-Ping, JIA Yong-Xia. Effects of Hypoxia Stress on Anaerobic Respiratory Enzyme and Antioxidant Enzyme Activities in Roots of Cucumber Seedlings[J]. Plant Science Journal, 2005, 23(4): 337-341.
    [7]QIU Chang-En, SHAO Jin-Zhen, CHEN Shao-Ying, YAO Fa-Xin. The Effect of 6-Benzyl Aminopurine on the Activities of Four Extracellular Enzymes in Plurotus ostreatus and Lentinula edodes Mycelium[J]. Plant Science Journal, 2002, 20(3): 233-235.
    [8]Zhang Chunlei, Zhang Rongxian. COMPARATIVE STUDIES ON ENZYMATIC KINETICS PARAMETERS OF RIBULOSE 1,5 BISPHOSPHATE CARBOXYLASE/OXYGENASE IN CHLAMYDOMONAS REINHARDTII[J]. Plant Science Journal, 1999, 17(2): 115-121.
    [9]Zhao Jie, Cheng Jingchen. EFFECTS OF LIGHT FACTORS ON SOLUBLE PROTEINS AND ENZYMES ACTIVITIES DURING GALLUS GROWTH OF ASPARGUS OFFICINALIS L.[J]. Plant Science Journal, 1994, 12(3): 251-257.
    [10]Li Xuebao. EFFECTS OF EMS ON THE GERMINATING SEEDS OF RICE[J]. Plant Science Journal, 1991, 9(3): 263-267.

Catalog

    Get Citation
    PDF
    XML
    Article views (1975) PDF downloads (1925) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles
    Copyright © 2022 Plant Science Journal 鄂ICP备05004779号-3
    Address：No. 201, Jiufeng 1st Road, Donghu High tech Zone, WuhanPostal Code：430074

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return