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ALITONG Qimike, WANG Qing-Feng, YANG Chun-Feng, CHEN Jin-Ming. ISSR Analysis on Genetic Diversity of Medically Important Lycium ruthenicum Murr.in Xinjiang[J]. Plant Science Journal, 2013, 31(5): 517-524. DOI: 10.3724/SP.J.1142.2013.50517
Citation: ALITONG Qimike, WANG Qing-Feng, YANG Chun-Feng, CHEN Jin-Ming. ISSR Analysis on Genetic Diversity of Medically Important Lycium ruthenicum Murr.in Xinjiang[J]. Plant Science Journal, 2013, 31(5): 517-524. DOI: 10.3724/SP.J.1142.2013.50517
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ISSR Analysis on Genetic Diversity of Medically Important Lycium ruthenicum Murr.in Xinjiang

  • 1.

    Department of Biological Engineering, Xinjiang Bayinguoleng Vocational and Technical College, Kuerle, Xinjiang 841000, China

  • 2. Xinjiang Bazhou Halixin Mongolian Medicinal Technology Development Co., Ltd, Kuerle, Xinjiang 841000, China

  • 3. Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China

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  • Received Date: January 10, 2013
  • Revised Date: March 14, 2013
  • Published Date: October 29, 2013
    Graphical Abstract
    • Abstract
  • Lycium ruthenicum (Solanaceae) is an important medicinal plant mainly distributed in Northwestern China.In recent years,however,the distribution range of this species has been reduced greatly due to human overexploitation and deterioration of its habitat.Studying the population genetics of this species is essential for the conservation and sustainable utilization of the plant germplasm resource.In this study,we used seven ISSR markers to measure genetic diversity of 115 individuals from eight natural populations of L.ruthenicum in Xinjiang (six populations) and Gansu (two populations).A total of 64 DNA fragments were scored,and 78.1% of polymorphic loci (PPL) found.High level of genetic diversity was detected in L.ruthenicum (H=0.29;Ⅰ=0.43).AMOVA analysis indicated that most variance (77.0%) resided within populations,with only a small proportion of total variation residing between populations (23%,FST=0.23).Results of the Mantel test (r=0.3602,p=0.910) and UPGMA cluster of the Xinjiang populations indicated that there was no significant relationship between geographical distance and genetic distance.UPGMA cluster of all sampled individuals showed that individuals from each population did not cluster together.The probable causes that have contributed to the current pattern of genetic structure were discussed and suggestions on future protection of the L.ruthenicum from southern Xinjiang were also given.
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