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FAN Jin-Ping, ZHAO Ran. Cloning and Expression Pattern Analysis of the ApNAP Gene in Asarina procumbens[J]. Plant Science Journal, 2014, 32(3): 251-258. DOI: 10.3724/SP.J.1142.2014.30251
Citation: FAN Jin-Ping, ZHAO Ran. Cloning and Expression Pattern Analysis of the ApNAP Gene in Asarina procumbens[J]. Plant Science Journal, 2014, 32(3): 251-258. DOI: 10.3724/SP.J.1142.2014.30251

Cloning and Expression Pattern Analysis of the ApNAP Gene in Asarina procumbens

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  • Received Date: December 11, 2013
  • Revised Date: March 11, 2014
  • Available Online: November 01, 2022
  • Published Date: June 29, 2014
  • Asarina procumbens, Scrophulariaceae, is a perennial vine and good ornamental plant for vertical greening in the garden. However, when the leaves of A.procumbens become dry and abscised after senescing, the bare tendrils greatly reduce its ornamental value. AtNAP is a NAC family transcription factor gene that plays a key role in regulating leaf senescence in Arabidopsis. In this study, ApNAP, a homologous gene of AtNAP, was cloned from A.procumbens senescent leaves by RT-PCR and RACE. Results showed that the ApNAP gene was located in the nucleus and had a total length of 1341 bp with an open reading frame of 936 bp, encoding 311 amino acids, of which the relative molecular weight was 35.21 kD and the isoelectric point was 9.13. The length of the genome was 1526 bp with two introns and three exons. Multiple sequence alignment indicated that the ApNAP gene was a NAC transcription factor. Phylogenetic analysis indicated that the ApNAP gene belonged to the NAP subfamily and shared highest similarity with AtNAM in Arabidopsis. The results of real-time qPCR showed that the expression level of ApNAP was increased under treatments induced by natural and dark-induced senescence, indicating that it was involved in the regulation of the leaf senescent process of A.procumbens. The isolation and cloning of ApNAP had an important value in delaying leaf senescence and improving the greening value of A.procumbens.
  • [1]
    张美恒, 胡翠平, 樊金萍. 金鱼藤的组织培养和快速繁殖体系的建立[J]. 作物杂志, 2012(2): 59-62.
    [2]
    Lim PO, Kim HJ, Nam HG. Leaf senescence[J]. Annu Rev Plant Biol,2007, 58: 115-136.
    [3]
    梁秋霞, 曹刚强, 苏明杰, 秦广雍. 植物叶片衰老研究进展[J]. 中国农学通报, 2006, 22(8): 282-285.
    [4]
    Gan S, Amasino RM. Making sense of senescence (molecular genetic regulation and manipulation of leaf senescence) [J]. Plant Physi, 1997, 113(2): 313-319.
    [5]
    Duval M, Hseieh AN, Skriver K. Molecular characterization of AtNAM: a member of the Arabidopsis NAC domain super family[J]. Plant Mol Biol, 2002, 50: 237-248.
    [6]
    Ooka H, Satoh K, Doi K. Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana[J]. DNA Res, 2003, 10(6): 239-247.
    [7]
    Souer E, Houwelingen A, Kloos D, Mol J, Koes R. The no apical meristem gene of Petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries[J]. Cell, 1996, 85: 159-170.
    [8]
    李伟, 韩蕾, 钱永强, 孙振元. 植物NAC转录因子的种类、特征及分类[J]. 应用与环境生物学报, 2011, 17(4): 596-606.
    [9]
    Ernst HA, Olsen AN, Skriver K, Larsen S, Leggio LL. Structure of the conserved domain of ANAC, a member of the NAC family of transcription factors[J]. EMBO Rep, 2004, 5 (3): 297-303.
    [10]
    Jensen MK, Rung JH, Gregersen PL, Gjetting T, Fuglsang AT, Hansen M, Joehnk N, Lyngkjaer MF, Collinge DB. The HvNAC6 transcription factor: A positive regulator of penetration resistance in barley and Arabidopsis[J]. Plant Mol Biol, 2007, 65 (1-2): 137-150.
    [11]
    He XJ, Mu RL, Cao WH, Zhang ZG, Zhang JS, Chen SY. AtNAC2,a transcription factor downstream of ethylene and auxin signaling pathways,is involved in salt stress response and lateral root development[J]. Plant J, 2005, 44: 903-916.
    [12]
    Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J. A NAC gene regulating senescence improves grain protein,zinc,and iron content in wheat[J]. Science, 2006, 314: 1298-1301.
    [13]
    Balazadeh S,Kwasniewski M, Caldana C. ORSI, an H2O2-responsive NAC transcription factor, controls senescence in Arabidopsis thaliana[J]. Mol Plant, 2011, 4(2): 346-360.
    [14]
    Guo YF, Gan S. AtNAP, a NAC family transcription factor has an important role in leaf senescence[J]. Plant J, 2006, 46(4): 601-612.
    [15]
    Mitsuda N, Iwase A, Yamamoto H, Yoshida M, Seki M, Shinozaki K, Ohme TM. The NAC transcription factors, NST1 and NST3, are key regulators of the formation of secondary walls in woody tissues of Arabidopsis[J]. Plant Cell, 2007, 19: 270-280.
    [16]
    Mitsuda N, Seki M, Shinozaki K, Ohme TM. The NAC transcription factors NST1 and NST2 of Arabidopsis regulates secondary wall thickening and are required for another dehiscence[J]. Plant Cell, 2005, 17: 2993-3006.
    [17]
    Zhong R, Demura T, Ye ZH. SND1, a NAC domain transcription factor, is a key regulator of secondary wall synthesis in fibers of Arabidopsis[J]. Plant Cell, 2006, 18: 3158-3170.
    [18]
    Yoo SY, Kim Y, Kim SY, Lee JS, Ahn JH. Control of flowering time and cold response by a NAC-domain protein in Arabidopsis[J]. PLoS One, 2007, 2(7): 637-642.
    [19]
    Nakashima K, Tran LP, Nguyen DV, Fujita M, Maruyama K, Todaka D, Ito Y, Hayashi N, Shinozaki K, Yamaguchi-Shinozaki K. Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress responsive gene expression in rice[J]. Plant J, 2007, 51: 617-630.
    [20]
    Sablowski RW, Meyerowitz EM. A homolog of NO APICAL MERISTEM is an immediate target of the floral homeotic genes APETALA3/PISTILLATA[J]. Cell, 1998, 92(1): 93-103.
    [21]
    Liu YZ, Baig RMN, Fan R, Ye JY. Identification and expression pattern of a novel NAM,ATAF,and CUC-Like gene from Citrus sinensis Osbeck[J]. Plant Mol Biol Rep, 2009, 27(3): 292-297.
    [22]
    Kalivas A, Pasentsis K, Arglriou A, Darentas N, Tsaftaris AS. FamRCA-RACE: a rolling circle amplification race for isolating a family of homologous cDNAs in one reaction and its application to obtain NAC genes transcription factors from crocus (Crocus sativus) flower[J]. Prep Bioehem Biotechnol, 2010, 40(3): 177-187.
    [23]
    唐雪蓉, 张如奎, 陈旭, 吴秀菊, 明凤. 水稻OsNAP基因的功能分析及其在育种价值方面的研究[J]. 复旦大学学报:自然科学版, 2012, 51(4): 506-514.
    [24]
    郭玉娟, 魏强, 蒯本科. 高羊茅中衰老相关转录因子AtNAP同源基因的分离及功能分析[J]. 复旦大学学报:自然科学版, 2010, 49(5): 544-553.
    [25]
    陈云霞. 竹子中叶片衰老相关基因的分离与功能分析[D]. 南京:南京林业大学,2011.
    [26]
    高俊凤. 植物生理学实验指导[M]. 北京:高等教育出版社,2006:5-6.
    [27]
    Kalivas A, Pasentsis K, Arglriou A, Darentas N, Tsaftaris AS. Isolation, characterization and expression analysis of an NAP-like cDNA from Crocus (Crocus sativus L.) [J]. Plant Mol Biol Rep, 2010, 28: 654-663.
    [28]
    Zhou Y, Huang WF, Liu L, Lin YJ. Identification and functional characterization of a rice NAC gene involved in the regulation of leaf senescence[J]. BMC Plant Biol, 2013,13: 132-144.
    [29]
    Zhang KW, Gan SS. An abscisic acid-AtNAP transcription factor-SAG113 protein phosphatase 2C regulatory chain for controlling dehydration in senescing Arabidopsis leaves[J]. Plant Physiol, 2012,158: 961-969.
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