Advance Search
YUE Lin, KUANG Yan-Feng, LIAO Jing-Ping. Quick Detection of Pollen Developmental Stages of Hamelia patens Jacq.[J]. Plant Science Journal, 2014, 32(4): 421-426. DOI: 10.3724/SP.J.1142.2014.40421
Citation: YUE Lin, KUANG Yan-Feng, LIAO Jing-Ping. Quick Detection of Pollen Developmental Stages of Hamelia patens Jacq.[J]. Plant Science Journal, 2014, 32(4): 421-426. DOI: 10.3724/SP.J.1142.2014.40421
shu

Quick Detection of Pollen Developmental Stages of Hamelia patens Jacq.

  • 1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;

  • 2. University of Chinese Academy of Sciences, Beijing 100049, China

More Information
  • Received Date: January 22, 2014
  • Revised Date: February 13, 2014
  • Available Online: November 01, 2022
  • Published Date: August 29, 2014
    Graphical Abstract
    • Abstract
  • DAPI is a DNA-specific fluorochrome that facilitates observation of nuclei during pollen development and can confirm the developmental stages of pollen grains. However, the nuclei in Hamelia patens Jacq. pollen grains are little stained by DAPI due to the resistance of the pollen wall. An effective "water-bath heating and oxidation" method was used to treat the pollen wall of H. patens. The results showed that the: (1) best incubating conditions for uninucleate microspores and bicellular pollen grains were 20~50 min at 65℃ and 20~40 min at 55℃, respectively; and, (2) lengths of flowers and anthers were 0.90~1.00 cm and 0.50~0.60 cm at the tetrad stage, 1.10~1.60 cm and 0.60~0.85 cm at the uninucleate microspore stage, and 1.80~2.70 cm and 0.91~1.01 cm at the bicellular pollen stage, respectively.
    • FullText(HTML)
    • References (15)
  • [1]
    朱骏, 杨仲南. 花粉壁发育研究进展[J]. 自然杂志, 2013, 35(2): 112-117.
    [2]
    Heslop-Harrison J. Pollen walls as adaptive systems[J]. Ann Mo Bot Gard, 1979, 66(4): 813-82.
    [3]
    Dessein S, Ochoterena H, Block PD, Lens F, Robbrecht E, Schols P, Smets E, Vinckier S, Huysmans S. Palynological characters and their phylogenetic signal in Rubiaceae[J]. Bot Rev, 2005, 71(3): 354-414.
    [4]
    朱澂, 林辰涛. 一种新型的DNA荧光染料DAPI的光学特性及其应用[J]. 武汉植物学研究, 1986, 4(1): 91-102.
    [5]
    Erickson RO. Modeling of plant growth[J]. Ann Rev Plant Physiol, 1976, 27: 407-34.
    [6]
    Gould KS, Lord EM. Growth of anthers in Lilium longiflorum[J]. Planta, 1988, 173(2): 161-171.
    [7]
    Honys D, Twell D. Transcriptome analysis of haploid male gametophyte development in Arabidopsis[J]. Genome Biol, 2004, 5(11): R85.
    [8]
    Deveshwar P, Bovill WD, Sharma R, Able JA, Kapoor S. Analysis of anther transcriptomes to identify genes contributing to meiosis and male gametophyte development in rice[J]. BMC Plant Biol, 2011, 11(1): 78.
    [9]
    Loewus FA, Baldi BG, Franceschi VR, Meinert LD, McCollum JJ. Pollen sporoplasts: dissolution of pollen walls[J]. Plant Physiol, 1985, 78(3): 652-654.
    [10]
    Tanaka I, Kitazume C, Ito M. The isolation and culture of lily pollen protoplasts[J]. Plant Sci, 1987, 50(3): 205-211.
    [11]
    周嫦. 三种植物花粉原生质体的大量分离与初步培养[J]. 植物学报, 1988, 30(4): 362-367.
    [12]
    吴旺泽, 王蒂, 王清, 彭晓莉. 马铃薯脱外壁花粉的制备与人工萌发[J]. 园艺学报, 2005, 32(1): 39-43.
    [13]
    冯九焕, 卢永根, 刘向东, 徐雪宾. 水稻花粉发育过程及其分期[J]. 中国水稻科学, 2001, 15(1): 21-28.
    [14]
    Zhang DB, Luo X, Zhu L. Cytological analysis and genetic control of rice anther development[J]. J Genet Genomics, 2011, 38(9): 379-390.
    [15]
    岳洁瑜, 吴李君, 吴跃进, 张宝龙, 唐灿明. 陆地棉脱外壁花粉粒的制备和花粉粒核 DNA 检测[J]. 棉花学报, 2008, 20(2): 94-98.
    • Related Articles

  • Related Articles

    [1]Yang Peng, Shu Jian-Feng, Cai Sha-Sha, Zhao Cai. nrDNA ITS sequence analysis and genetic relationship of Allium macrostemon from different geographical regions in Guizhou[J]. Plant Science Journal, 2017, 35(2): 171-176. DOI: 10.11913/PSJ.2095-0837.2017.20171
    [2]ZHANG Xiao-Xia, XIONG Xian-Hua, XU Fang, DENG Li-Na, WANG Kang-Man, HOU Yuan-Tong. Exocarp Anatomy of Polygonum and Related Species from Shandong Province[J]. Plant Science Journal, 2011, 1(5): 544-551.
    [3]XU Chong-Mei, QU Chang-You, YU Wen-Guang, ZHANG Xue-Jie, LI Fa-Zeng. Phylogenetic Origin of Polygonum perfoliatum Inferred from Plastid trnL-F Sequences[J]. Plant Science Journal, 2009, 27(3): 262-265.
    [4]DING Li, MENG Ai-Ping, LI Jian-Qiang. Meiosis Observation of Rumex japonicus Houtt.(Polygonaceae)[J]. Plant Science Journal, 2009, 27(3): 242-245.
    [5]LI Bo, CHEN Shao-Feng, ZHANG Wen-Gen. A New Variety of Polygonum from Jiangxi and Its Morphological Characters of Leaf Epidermis[J]. Plant Science Journal, 2008, 26(1): 38-40.
    [6]LIU Yan-Ling, WU Jian-Ming, XU Li-Ming, ZHAO JiaRong, ZHAO Zi-En. Polygonum chinense var.procumbens Z.E.Zhao et J.R.Zhao(Polygonaceae), A New Variety from Hainan[J]. Plant Science Journal, 2007, 25(6): 561-562.
    [7]XUE Hua-Jie, WANG Nian-He, LU Chang-Mei, SUN Ming-Yi, MASAHIKO Taniguch, KIMIE Baba. Phylogenetic Position of Anegelica apaensis/Heracleum apaense Based on Evidence from ITS Sequences of Nuclear Ribosomal DNA[J]. Plant Science Journal, 2007, 25(2): 143-148.
    [8]ZHU Li-Tao, LU Fa-Jun, HOU Yuan-Tong, LI Fa-Zeng. Micro-morphology of Leaf Epidermis and Its Taxonomical Significance of Polygonum Section Cephalophilon in China[J]. Plant Science Journal, 2007, 25(2): 136-142.
    [9]HOU Yuan-Tong, QU Chang-You, LU Fa-Jun, LI Fa-Zeng. The Pollen Morphology of the genus Polygonum s.str.(Polygonaceae)in China and its Classific Significance[J]. Plant Science Journal, 2007, 25(2): 127-135.
    [10]Chen Changbin, Feng Zhijian. STUDY ON THE POLLEN MORPHOLOGY OF THE GENUS RUMEX IN CHINA[J]. Plant Science Journal, 1996, 14(1): 16-24.

Catalog

    Get Citation
    PDF
    XML
    Article views (1102) PDF downloads (1863) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles
    Copyright © 2022 Plant Science Journal 鄂ICP备05004779号-3
    Address：No. 201, Jiufeng 1st Road, Donghu High tech Zone, WuhanPostal Code：430074

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return