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Wagutu Godfrey Kinyori, Njeri Henry Kariuki, Fan Xiang-Rong, Chen Yuan-Yuan. Development and transferability analysis of SSR primers in wild rice Zizania latifolia (Poaceae)[J]. Plant Science Journal, 2020, 38(1): 105-111. DOI: 10.11913/PSJ.2095-0837.2020.10105
Citation: Wagutu Godfrey Kinyori, Njeri Henry Kariuki, Fan Xiang-Rong, Chen Yuan-Yuan. Development and transferability analysis of SSR primers in wild rice Zizania latifolia (Poaceae)[J]. Plant Science Journal, 2020, 38(1): 105-111. DOI: 10.11913/PSJ.2095-0837.2020.10105
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Development and transferability analysis of SSR primers in wild rice Zizania latifolia (Poaceae)

  • 1. Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;

  • 2. University of Chinese Academy of Sciences, Beijing 100049, China;

  • 3. Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China;

  • 4. College of Science, Tibet University, Lhasa 850000, China;

  • 5. Research Center for Ecology and Environment of Qinghai-Tibetan Plateau, Tibet University, Lhasa 850000, China

Funds: 

This work was supported by a grant from the Talent Program of Wuhan Botanical Garden, Chinese Academy of Sciences (Y855291B01).

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  • Received Date: April 01, 2019
  • Revised Date: June 14, 2019
  • Available Online: October 31, 2022
  • Published Date: February 27, 2020
    Graphical Abstract
    • Abstract
  • Zizania latifolia Turcz., also known as Manchurian wild rice, is a member of the tribe Oryzeae, and a major wild ecological and genetic resource. In this study, nuclear SSR primers were developed in silico based on part of the existing genome sequences of Z. latifolia. Five wild populations of the species from different regions across China were selected to screen 64 developed primers. Results showed that 15 primer pairs were polymorphic in at least one population. In addition, we identified a total of 84 alleles, with an average of 5.6 alleles per locus. For the different populations, the level of observed and expected heterozygosity ranged from 0.000 to 0.941 and 0.072 to 0.625, respectively. Relatively high genetic differentiation between populations (FST=0.432) was found, as evidenced by low levels of gene flow (Nm=0.576) among populations. These newly developed markers will facilitate further study of the level and pattern of genetic diversity, and the development of germplasm resource conservation strategies for natural extant Z. latifolia populations. In the cross-species transferability test, eight and nine of the 15 loci were successfully amplified in Oryza sativa L. and O. rufipogon Griff., respectively.
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    • References (21)
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