Relationships between endophytic structure, rhizosphere soil properties and aconite alkaloids accumulations in <i>Aconitum carmichaelii</i> Debx.

QIU Hao; CHEN Jia-yang; LAI You-zhen; ZHANG Guo-yan; CUI Lang-jun; WEI Xi-ying

doi:10.11913/PSJ.2095-0837.2021.60643

Plant Science Journal > 2021 > 39(6): 643-653. > DOI: 10.11913/PSJ.2095-0837.2021.60643 CSTR: 32231.14.PSJ.2095-0837.2021.60643

QIU Hao, CHEN Jia-yang, LAI You-zhen, ZHANG Guo-yan, CUI Lang-jun, WEI Xi-ying. Relationships between endophytic structure, rhizosphere soil properties and aconite alkaloids accumulations in Aconitum carmichaelii Debx.[J]. Plant Science Journal, 2021, 39(6): 643-653. DOI: 10.11913/PSJ.2095-0837.2021.60643

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Relationships between endophytic structure, rhizosphere soil properties and aconite alkaloids accumulations in Aconitum carmichaelii Debx.

1. College of Life Science, National Engineering Laboratory of Resources Development of Endangered Medicinal Materials in Northwest China, Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry of Ministry of Education, Shaanxi Normal University, Xi'an 710119, China;
2. Chenggu County Qunli Traditional Chinese Medicine Cooperative, Hanzhong, Shaanxi 723200, China

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supported by grants from the National Key R&D Plan(2012GS610102)

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Received Date: November 08, 2020
Revised Date: March 14, 2021
Available Online: October 31, 2022
Published Date: December 27, 2021

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Abstract

Abstract

In this study, Aconitum carmichaelii Debx. and rhizosphere soil from different areas were studied. The changes of endophytic microorganisms, soil microorganisms, alkaloid content and soil element factors of Aconitum carmichaelii were studied by illuminahiseq high-throughput sequencing technology and high performance liquid chromatography. The results showed that: the contents of benzoylneaconitine, benzoylaconitine and monoester alkaloids were positively correlated with Acinetobacter abundance, soil pH and Al³⁺ content，while negatively with Aspergillus abundance. The content of hypoaconitine was positively correlated with Bradyrhizobium abundance. However, the other two alkaloids (benzoylsubaconitine and hypaconitine) accumulations were negatively correlated with Athelia and Fusarium abundances. The results show that: changing the abundances of the key microorganism (such as Bradyrhizobium, Acinetobacter, Athelia, Fusarium and Aspergillus), and improving the Al³⁺ contents and pH in rhizosphere soil probably increase the contents of several alkaloids in Aconitum carmichaelii field management programs.
- Aconite,
- Alkaloid,
- Endophyte,
- Microbial community

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References

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Relationships between endophytic structure, rhizosphere soil properties and aconite alkaloids accumulations in Aconitum carmichaelii Debx.

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