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Huang Y,Zhai L,Xie LL,Xu JS,Zhang XK,Xu BB. Genome-wide identification and expression analysis of the MAP70 gene family in Brassica napus L.[J]. Plant Science Journal,2023,41(5):647−656. doi: 10.11913/PSJ.2095-0837.22245
Citation: Huang Y,Zhai L,Xie LL,Xu JS,Zhang XK,Xu BB. Genome-wide identification and expression analysis of the MAP70 gene family in Brassica napus L.[J]. Plant Science Journal,2023,41(5):647−656. doi: 10.11913/PSJ.2095-0837.22245

Genome-wide identification and expression analysis of the MAP70 gene family in Brassica napus L.

  • MAP70 family members were screened and identified using bioinformatics based on the genomic database of Brassica napus L., with bioinformatics analysis performed on the sequence characteristics of the identified BnaMAP70 gene family members. Expression levels of the BnaMAP70 genes were analyzed using qRT-PCR in seedling varieties with waterlogging sensitivity and tolerance. In total, 19 members of the BnaMAP70 gene family were identified, distributed on 11 chromosomes and divided into five subfamilies. The upstream elements involved in anaerobic stress and phytohormones of the BnaMAP70 promoter indicated that they may be involved in plant growth, development, and regulation of waterlogging stress. The transcription levels of genes were species-specific, plant site-specific, and time-specific. The qRT-PCR results showed that BnaMAP70-1 and BnaMAP70-4 were differentially expressed under waterlogging stress, indicating that these two genes may be regulated by waterlogging stress.
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