| Citation: |
Wang J，Zhang T，Wei AL，Wang QH，Shi JQ，Wang ZJ. A new record of a filamentous cyanobacterial species in China: Oculatella ucrainica Oxana Vinogradova & Tatiana Mikhailyuk[J]. Plant Science Journal，2025，43（1）：1−10. DOI: 10.11913/PSJ.2095-0837.24024
|
A newly recorded drought-tolerant terrestrial cyanobacterium, Oculatella ucrainica Oxana Vinogradova & Tatiana Mikhailyuk, originally isolated from soil crusts and coastal areas in 2017, is reported in China for the first time. In this study, two filamentous cyanobacteria were isolated from moist soil at Taiyuan Normal University, Shanxi Province, and damp walls in Huaxi Park, Guizhou Province. These strains displayed morphological similarities to O. ucrainica, both featuring orange spots on the apical cells of their trichomes. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated high similarity (above 99%) between the two isolates and O. ucrainica, with both strains clustering on a single branch with high bootstrap support. Furthermore, the secondary structure of these cyanobacteria closely resembled that of O. ucrainica, further corroborating their identification as members of this species. This study represents the first report of O. ucrainica in China.
| [1] |
Fritsch FE. Kryptogamen-flora von deutschland,österreich und der schweiz[J]. Nature,1932,130(3278):292−293. doi: 10.1038/130292a0
|
| [2] |
Schirrmeister BE,Antonelli A,Bagheri HC. The origin of multicellularity in cyanobacteria[J]. BMC Evol Biol,2011,11(1):45. doi: 10.1186/1471-2148-11-45
|
| [3] |
Herrero A,Stavans J,Flores E. The multicellular nature of filamentous heterocyst-forming cyanobacteria[J]. FEMS Microbiol Rev,2016,40(6):831−854. doi: 10.1093/femsre/fuw029
|
| [4] |
Komárek J. Problem of the taxonomic category "species" in cyanobacteria[J]. Algol Stud,2003,109(1):281−297.
|
| [5] |
谢艳,张荣臻,张葵东,吕兴菊,张和,等. 中国水华蓝藻新记录属——泽鞘丝藻属(Limnoraphis)[J]. 河南师范大学学报(自然科学版),2023,51(4):125−131.
Xie Y,Zhang RZ,Zhang KD,Lü XJ,Zhang H, et al. Limnoraphis (Oscillatoriales,Cyanobacteria),a bloom-forming genus newly recorded from China[J]. Journal of Henan Normal University (Natural Science Edition),2023,51(4):125−131.
|
| [6] |
Komárek J. A polyphasic approach for the taxonomy of cyanobacteria:principles and applications[J]. Eur J Phycol,2016,51(3):34−353.
|
| [7] |
魏爱丽,杨谢,王捷,巩超彦,王清华,李艳晖. 念珠藻类(蓝藻)psbA基因的进化分析[J]. 河南师范大学学报(自然科学版),2023,51(1):114−122.
Wei AL,Yang X,Wang J,Gong CY,Wang QH,Li YH. Evolutionary analysis on psbA gene of Nostoc-like (Cyanobacteria)[J]. Journal of Henan Normal University (Natural Science Edition),2023,51(1):114−122.
|
| [8] |
Geitler L,Ruttner F. Die cyanophyceen der Deutschen limnologischen Sunda-expedition,ihre morphologie,systematik und ökologie. zweiter teil[J]. Arch Hydrobiol Trop Binnengewä sser,1936,14:371−483.
|
| [9] |
Komárek J,Kaštovský J,Mareš J,Johansen JR. Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014,using a polyphasic approach[J]. Preslia,2014,86(4):295−335.
|
| [10] |
Strunecký O,Ivanova AP,Mareš J. An updated classification of cyanobacterial orders and families based on phylogenomic and polyphasic analysis[J]. J Phycol,2023,59(1):12−51. doi: 10.1111/jpy.13304
|
| [11] |
Zammit G,Billi D,Albertano P. The subaerophytic cyanobacterium Oculatella subterranea (Oscillatoriales,Cyanophyceae)gen. et sp. nov.:a cytomorphological and molecular description[J]. Eur J Phycol,2012,47(4):341−354. doi: 10.1080/09670262.2012.717106
|
| [12] |
Vinogradova O,Mikhailyuk T,Glaser K,Holzinger A,Karsten U. New species of Oculatella (Synechococcales,Cyanobacteria) from terrestrial habitats of Ukraine[J]. Ukr Bot J,2017,74(6):509−520. doi: 10.15407/ukrbotj74.06.509
|
| [13] |
Kim M,Oh HS,Park SC,Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes[J]. Int J Syst Evol Microbiol,2014,64(Pt 2):346−351.
|
| [14] |
Rippka R. Recognition and identification of cyanobacteria[J]. Methods Enzymol,1988,167:28−67.
|
| [15] |
Pachiappan P,Prasath BB,Perumal S,Ananth S,Devi AS, et al. Isolation and culture of microalgae[M]//Perumal S,Thirunavukkarasu AR,Pachiappan P,eds. Advances in Marine and Brackishwater Aquaculture. New Delhi:Springer,2015:1−15.
|
| [16] |
Van Ranst G,Baert P,Fernandes AC,de Grave J. Technical note:Nikon-TRACK Flow,a new versatile microscope system for fission track analysis[J]. Geochronology,2020,2(1):93−99. doi: 10.5194/gchron-2-93-2020
|
| [17] |
Fiore MF,Moon DH,Tsai SM,Lee H,Trevors JT. Miniprep DNA isolation from unicellular and filamentous cyanobacteria[J]. J Microbiol Methods,2000,39(2):159−169. doi: 10.1016/S0167-7012(99)00110-4
|
| [18] |
Neilan BA,Jacobs D,Goodman AE. Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus[J]. Appl Environ Microbiol,1995,61:3875−3883. doi: 10.1128/aem.61.11.3875-3883.1995
|
| [19] |
Edwards U,Rogall T,Blöcker H,Emde M,Böttger EC. Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA[J]. Nucleic Acids Res,1989,17(19):7843−7853. doi: 10.1093/nar/17.19.7843
|
| [20] |
Lin S,Wu ZX,Yu GL,Zhu ML,Yu BS,Li RH. Genetic diversity and molecular phylogeny of Planktothrix (Oscillatoriales,cyanobacteria) strains from China[J]. Harmful Algae,2010,9(1):87−97. doi: 10.1016/j.hal.2009.08.004
|
| [21] |
Turner S,Pryer KM,Miao VPW,Palmer JD. Investigating deep phylogenetic relationships among cyanobacteria and plastids by small subunit rRNA sequence analysis[J]. J Eukaryotic Microbiol,1999,46(4):327−338. doi: 10.1111/j.1550-7408.1999.tb04612.x
|
| [22] |
Iteman I,Rippka R,de Marsac NT,Herdman M. Comparison of conserved structural and regulatory domains within divergent 16S rRNA-23S rRNA spacer sequences of cyanobacteria[J]. Microbiology,2000,146(6):1275−1286. doi: 10.1099/00221287-146-6-1275
|
| [23] |
张军毅,孙蓓丽,朱冰川,石浚哲,周克茹,等. 基于分子标记的藻类鉴定研究进展[J]. 湖泊科学,2021,33(6):1607−1625. doi: 10.18307/2021.0601
Zhang JY,Sun BL,Zhu BC,Shi JZ,Zhou KR,et al. Research progress in the taxonomic identification of algae on the basis of molecular markers[J]. Journal of Lake Sciences,2021,33(6):1607−1625. doi: 10.18307/2021.0601
|
| [24] |
肖喆,王捷,石瑛,王清华,李艳晖,谭梅娟. 晋阳湖丝状蓝藻的形态学分析与分子鉴定[J]. 西北植物学报,2022,42(3):427−434.
Xiao Z,Wang J,Shi Y,Wang QH,Li YH,Tan MJ. Morphological analysis and molecular identification of filamentous cyanobacteria from Jinyang Lake[J]. Acta Botanica Boreali-Occidentalia Sinica,2022,42(3):427−434.
|
| [25] |
彭焕文,王伟. 基于分子数据的系统发生树构建[J]. 植物学报,2023,58(2):261−273.
Peng HW,Wang W. Phylogenetic tree reconstruction based on molecular data[J]. Chinese Bulletin of Botany,2023,58(2):261−273.
|
| [26] |
Tamura K,Stecher G,Kumar S. MEGA11:molecular evolutionary genetics analysis version 11[J]. Mol Biol Evol,2021,38(7):3022−3027. doi: 10.1093/molbev/msab120
|
| [27] |
Stamatakis A. RAxML version 8:a tool for phylogenetic analysis and post-analysis of large phylogenies[J]. Bioinformatics,2014,30(9):1312−1313. doi: 10.1093/bioinformatics/btu033
|
| [28] |
Kalyaanamoorthy S,Minh BQ,Wong TKF,von Haeseler A,Jermiin LS. ModelFinder:fast model selection for accurate phylogenetic estimates[J]. Nat Methods,2017,14(6):587−589. doi: 10.1038/nmeth.4285
|
| [29] |
Hoang DT,Chernomor O,von Haeseler A,Minh BQ,Vinh LS. UFBoot2:improving the ultrafast bootstrap approximation[J]. Mol Biol Evol,2018,35(2):518−522. doi: 10.1093/molbev/msx281
|
| [30] |
Ronquist F,Huelsenbeck JP. MrBayes 3:Bayesian phylogenetic inference under mixed models[J]. Bioinformatics,2003,19(12):1572−1574. doi: 10.1093/bioinformatics/btg180
|
| [31] |
Pinto AR,Machado Cunha I,Rebelo Gomes E. Fig tree-induced phytophotodermatitis:a case report on the perils of a hobby[J]. Cureus,2023,15(7):e41888.
|
| [32] |
Reuter JS,Mathews DH. RNAstructure:software for RNA secondary structure prediction and analysis[J]. BMC Bioinformatics,2010,11:129. doi: 10.1186/1471-2105-11-129
|
| [33] |
Komárek J. Several problems of the polyphasic approach in the modern cyanobacterial system[J]. Hydrobiologia,2018,811(1):7−17. doi: 10.1007/s10750-017-3379-9
|
| [34] |
胡鸿钧,魏印心. 中国淡水藻类:系统、分类及生态[M]. 北京:科学出版社,2006:23−27.
|
| [35] |
黄冰心,丁兰平. 中国海洋蓝藻门新分类系统[J]. 广西科学,2014,21(6):580−586.
Huang BX,Ding LP. The catalogue of marine cyanobacteria of China[J]. Guangxi Sciences,2014,21(6):580−586.
|
| [36] |
Cai FF,Yu GL,Li RH. Description of two new species of Pseudoaliinostoc (Nostocales,Cyanobacteria) from China based on the polyphasic approach[J]. J Oceanol Limnol,2022,40(3):1233−1244. doi: 10.1007/s00343-021-1111-0
|
| [37] |
Mai T,Johansen JR,Pietrasiak N,Bohunická M,Martin MP. Revision of the Synechococcales(Cyanobacteria) through recognition of four families including Oculatellaceae fam. nov. and Trichocoleaceae fam. nov. and six new genera containing 14 species[J]. Phytotaxa,2018,365(1):1−59. doi: 10.11646/phytotaxa.365.1.1
|
| [38] |
李仁辉. 建议将Pseudanabaena译成“假鱼腥藻属”而不是“伪鱼腥藻属”[J]. 湖泊科学,2022,34(4):1392.
|
| [39] |
Osorio-Santos K,Pietrasiak N,Bohunická M,Miscoe LH,Kováčik L,et al. Seven new species of Oculatella (Pseudanabaenales,Cyanobacteria):taxonomically recognizing cryptic diversification[J]. Eur J Phycol,2014,49(4):450−470. doi: 10.1080/09670262.2014.976843
|
| [1] | Xiang Niyan, Chen Xitang, Yang Tiange, Liu Xihong, Liu Hong. Chrysosplenium fallax Koldaeva (Chrysosplenium), a newly recorded species from China[J]. Plant Science Journal, 2024, 42(4): 423-430. DOI: 10.11913/PSJ.2095-0837.23303 |
| [2] | Wang Meng-Hao, Ran Hang, Liu Yan-Yan, Sun Hua-Yue, Cao Ya-Nan, Wang Hong-Wei, Li Jia-Mei. Comparative chloroplast genomic and phylogenetic analysis of Aralia and related species[J]. Plant Science Journal, 2023, 41(2): 149-158. DOI: 10.11913/PSJ.2095-0837.22161 |
| [3] | Cao Guan-Long, Zou Dian-Yang, Zhou Run, Li Lang, Li Jie. A study on the phylogeny and species diversity of the genus Cryptocarya in China[J]. Plant Science Journal, 2021, 39(4): 349-357. DOI: 10.11913/PSJ.2095-0837.2021.40349 |
| [4] | Zhang Cai-Fei, Peng Shuai, Tian Jing, Hu Guang-Wan, Wang Qing-Feng. A new species and a newly recorded species of Impatiens (Balsaminaceae) from Yunnan, China[J]. Plant Science Journal, 2020, 38(4): 437-447. DOI: 10.11913/PSJ.2095-0837.2020.40437 |
| [5] | Liu Zhi-E, Wang Chun-Hui, Liu Wei-Qi, Wang Xiao-Fan. Molecular phylogeny of Armeniaca based on nuclear and chloroplast gene sequences: Exploring the origin and genetic relationship of Armeniaca hongpingensis[J]. Plant Science Journal, 2018, 36(5): 633-641. DOI: 10.11913/PSJ.2095-0837.2018.50633 |
| [6] | ZHAO Zhi-Juan, LIU Guo-Xiang, HU Zheng-Yu. A New Combination Species of Freshwater Cladophorales and Its Phylogenetic Analysis[J]. Plant Science Journal, 2015, 33(3): 281-290. DOI: 10.11913/PSJ.2095-0837.2015.30281 |
| [7] | LIU Xiu-Zhen, ZHANG Feng, ZHANG Jin-Tun. Study on Species Diversity during the Succession of Plant Communities on Abandoned Farmlands in Guancen Mountains,Shanxi[J]. Plant Science Journal, 2008, 26(4): 391-396. |
| [8] | Wang Qichao, Zhang Xingyan, Hu Chungen. NEW SYSTEM FOR LOTUS VARIETY CLASSIFICATION[J]. Plant Science Journal, 1997, 15(1): 19-26. |
| [9] | Xu Kexue. A NEW ALGORITHM FOR CLADISTIC TAXONOMY——EVOLUTIONARY EXTREMAL AGGREGATION METHOD[J]. Plant Science Journal, 1993, 11(3): 223-231. |
| [10] | Yang Yongkang, Wu Jiakun, Shao Jianzhang, Zhang Shuxian. NEW TAXA OF GENUS FRITILLARIA L. FROM CHINA (CONT.)[J]. Plant Science Journal, 1987, 5(2): 125-146. |
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: