Analysis of Electrophoretic Pattern of Seed Protein of Four Cultivated Species in Cucurbita

Using polyacrylamide gel electrophoresis(PAGE)to anlysis seed protein ingredients of 12 cultivars from four cultivated species in genus Cucurbita:C.moschato Duch,C.maxima Duch,C.pepo L.,C.ficifolia.The results showed:Among seed proteins extracted with water,0.5 mol·L^-1 NaCl,0.1 mol·L^-1 Lacetic acid and 70% Ethanol,most of them are salt-soluble proteins in C.ficifolia;In the other three cultivated species,most of them are water-soluble proteins,with little salt-soluble proteins;All of the four species have very little acid-soluble proteins;In the examination,no alcohol-soluble protein bandings were obtained.The maps of water-soluble proteins,salt-soluble proteins,acid-soluble proteins showed great difference among the four species,but there were almost no difference among the cultivars of the same specie.