Isolation of a New Low-molecular-weight Glutenin Subunit Gene in Wheat

PCR was used as a rapid alternative method to isolate allelic of wheat low molecular weight subunit(LMW-GS) genes.The primers specific to Glu-D3 locus were designed based on published LMW-GS gene sequence data.The PCR products were inserted into pUCm-T vector and transferred into E.coli DH5α.The size of PCR product was about 1287 bp,including partial promoter and whole coding sequences.The LMW-GS encoded by cloned gene in Xinjiang wheat,Rikeze,contained 8 Cys residuces,being grouped into TypeⅠ.This gene clone was applied in the gene function research by the method of gene engineering.