Cloning and Analyzing of Partial cDNA Sequences of FIE in Autonomous Endosperm Type Eulaliopsis binata

RNA was extracted from inflorescence of Eulaliopsis binata and used as template for cloning by RT-PCR with degenerate primers which were designed according to the FIE homologous sequences of Arabidopsis,rice and maize.As a result,one about 800 bp fragment was obtained and then cloned and sequenced.Analysis with partial cDNA sequence and deduced amino acid sequence suggested that the fragment was similar to the reported FIE homologous genes of maize,sorghum and rice.The partial cDNA is a part of FIE gene in Eulaliopsis binata.