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ZHI Li-Feng, YU Tao, PENG Mao-Min, ZHU Ying-Guo, LI Yang-Sheng. Cloning and Characterization of Novel Rice Cell cDNAs Under Iron-deficiency by Improved DDRT-PCR Method[J]. Plant Science Journal, 2005, 23(1): 7-10.
Citation: ZHI Li-Feng, YU Tao, PENG Mao-Min, ZHU Ying-Guo, LI Yang-Sheng. Cloning and Characterization of Novel Rice Cell cDNAs Under Iron-deficiency by Improved DDRT-PCR Method[J]. Plant Science Journal, 2005, 23(1): 7-10.

Cloning and Characterization of Novel Rice Cell cDNAs Under Iron-deficiency by Improved DDRT-PCR Method

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  • Received Date: April 01, 2004
  • Revised Date: June 01, 2004
  • Published Date: February 27, 2005
  • An improved differential display reverse transcript PCR(DDRT-PCR) approach was used to identify novel genes treated by iron-deficiency from rice cells.Total eight differential fragments(IDR1-8) were identified.Further northern blotting indicated that five of them were positive;three of those positive fragments were suppressed in iron-deficiency condition,while two of them were induced under iron-deficiency.Sequence analysis revealed that three of them (IDR2,IDR3,IDR8) represent novel genes.Two of them (IDR2,IDR8) were unknown genes,while the IDR3 was homogenous to some known genes by blasting approach.
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