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ZHANG Chuan-Jun, XIAO Ya-Ping, WANG Xiao-An. Establishment of FISH-AFLP System in Larix chinensis[J]. Plant Science Journal, 2007, 25(6): 619-623.
Citation: ZHANG Chuan-Jun, XIAO Ya-Ping, WANG Xiao-An. Establishment of FISH-AFLP System in Larix chinensis[J]. Plant Science Journal, 2007, 25(6): 619-623.
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Establishment of FISH-AFLP System in Larix chinensis

  • College of Life Sciences, Shaanxi Normal University, Xi’an 710062, China

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  • Received Date: April 22, 2007
  • Revised Date: August 28, 2007
  • Published Date: December 28, 2007
    Graphical Abstract
    • Abstract
  • The endosperm,dry leaves and fresh leaves of Larix chinensis were used as materials.By extracting DNA with an improved method of CTAB,digesting DNA with Mse I and EcoR I,pre-selective amplification,selective amplification,PAGE.FISH-AFLP technical system to detect genetic diversity in L.chinensis was established successfully.With primer combinations E-ACG/M-CAC and E-ACG/M-CAA,1856 and 2013 AFLP fragments were detected in during 28 individuals.Stable and abundant bands can be obtained with the materials of endosperms and fresh leaves.The techniques could be applied in the further analysis of genetic diversity in L.chinensis.
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