Effects of Arabinogalactan Proteins on Pollen Germination and Pollen Tube Growth of Torenia fourieri and Nicotiana tabacum L.

Effects of arabinogalactan proteins in pollen germination and pollen tube growth of Torenia fourieri and Nicotiana tabacum were studied with the in vitro pollen tube growth system.The results showed that the frequency of pollen germination and the speed of pollen tube growth of Torenia fourieri were decreased by the treatment of βGlcY(β-Glucosyl Yariv reagent,a regent specifically bind AGPs).The growth of tobacco pollen tubes was also inhibited by the provision of βGlcY to the medium.With the immunocytochemistry localization techniques,it was observed that AGPs were mainly distributed in the apertures of pollen grains.When the pollen tubes germinated,AGPs were distributed throughout the whole pollen tubes uniformly in N.tabacum L.,but absent in the pollen tubes of Torenia fourieri.