CHANGES ON THE HISTOCHEMICAL LOCALIZATION IN THE DEVELOPMENT PROCESS OF PROEMBRYO OF KETELEERIA EVELYNIANA MAST

A primary observation on the DNA, RNA, basic protein, acid protein and polysaccharide at the process of fertilization in the proembryo and early young embryo of Keteleeria evelyniana Mast has been made through histochemical methods. The following results are obtained:The DNA staining reaction was weaker by using Feulgen reation before and after fertilization within the egg nucleus. After formed proembryo and early young embryo, the content of DNA in the nuclei of the initials became normal. The RNA and acid protein contents are rich during the whole development, especially within the embryo initials. However, A-S staining of the basic protein was negative within the neocytoplasm, but the DNA, RNA, acid protein and polysaccharide were positive.