Allophycocyanin Purification from Porphyra haitanensis and Studies on Spectrum of Eluting Fractions

Allophycocyanin from Porphyra haitanensis collected in Pingtan County of Fujian Province was purified using ammonium sulfate precipitation and column chromatography.Meanwhile,factors of purification were studied in detail.The results showed that using 30%-35% of the saturation ammonium sulfate precipitation,Tris-HCl(pH=8.0)as eluting buffering and DEAE-Sephadex-A-50 as chromatographic adsorbent;the purity and rate of recovery of allophycocyanin obtained were 3.50 and 70.2%,(respectively).SDS-PAGE demonstrated the presence of two subunits,α and β,with Mr(18.9 kD) and(17.7 kD),respectively.Therefore the method was suitable for quick purification of allophycocyanin from P.haitanensis.At the same time,the fractions collected from column were researched by analysis of spectrum.The findings indicated that P.haitanensis contained the R-phycoerythrin with two absorption peaks which had the polypeptide composition(αβ)_6γ,the R-phycocyanin which had the polypeptide composition(αβ)₃ and allophycocyanin-II which had the polypeptide composition(αβ)₃.