Construction and Identification of a cDNA Library for the Flower buds of Epimedium brevicornu Maxim

A cDNA library for flower buds of Epimedium brevicornu Maxim was constructed which based on SMART technology.Our results showed that the titer of unamplifed library from flower buds was 2×10⁶ pfu/mL.The percentage of recombinants was 95% and the average length of the insert cDNA fragments was 1095 bp.It suggested that one high quality cDNA library for flower buds of E.brevicornu were successfully constructed.Construction of cDNA library will help to clone the relevant genes associated with secondary metabolism,first,the special genes in the course of flavonoid biosynthesis;second,the genes related with development of flower buds in E.brevicornu Maxim.