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JIANG Tian-Liang, LI Yun-Xiang, YANG Zi-Song, HAN Su-Ju, CHEN Guang-Deng. Construction and Identification of a cDNA Library for the Flower buds of Epimedium brevicornu Maxim[J]. Plant Science Journal, 2007, 25(1): 105-108.
Citation: JIANG Tian-Liang, LI Yun-Xiang, YANG Zi-Song, HAN Su-Ju, CHEN Guang-Deng. Construction and Identification of a cDNA Library for the Flower buds of Epimedium brevicornu Maxim[J]. Plant Science Journal, 2007, 25(1): 105-108.
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Construction and Identification of a cDNA Library for the Flower buds of Epimedium brevicornu Maxim

  • 1.

    Sichuan Provincial Key Laboratory of Environmental Science and Biological Diversity Conservation, China West Normal University, Nanchong, Sichuan 637002, China

  • 2.

    Aba Teachers College, Wenchuan, Sichuan 623000, China

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  • Received Date: June 06, 2006
  • Revised Date: August 13, 2006
  • Published Date: February 12, 2007
    Graphical Abstract
    • Abstract
  • A cDNA library for flower buds of Epimedium brevicornu Maxim was constructed which based on SMART technology.Our results showed that the titer of unamplifed library from flower buds was 2×106 pfu/mL.The percentage of recombinants was 95% and the average length of the insert cDNA fragments was 1095 bp.It suggested that one high quality cDNA library for flower buds of E.brevicornu were successfully constructed.Construction of cDNA library will help to clone the relevant genes associated with secondary metabolism,first,the special genes in the course of flavonoid biosynthesis;second,the genes related with development of flower buds in E.brevicornu Maxim.
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