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HUANG Wen-Kun, GUO Jian-Ying, WAN Fang-Hao, GAO Bi-Da, XIE Bing-Yan. Genomic DNA Extraction Method and AFLP Amplification Reaction System of Ageratina adenophora Spreng.[J]. Plant Science Journal, 2006, 24(6): 498-504.
Citation: HUANG Wen-Kun, GUO Jian-Ying, WAN Fang-Hao, GAO Bi-Da, XIE Bing-Yan. Genomic DNA Extraction Method and AFLP Amplification Reaction System of Ageratina adenophora Spreng.[J]. Plant Science Journal, 2006, 24(6): 498-504.
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Genomic DNA Extraction Method and AFLP Amplification Reaction System of Ageratina adenophora Spreng.

  • 1.

    State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China

  • 2.

    Center for Management of Invasive Alien Species, Ministry of Agriculture, P.R.China, Beijing 100081

  • 3.

    College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha 410128, China

  • 4.

    Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

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  • Received Date: April 27, 2006
  • Revised Date: July 19, 2006
  • Published Date: December 27, 2006
    Graphical Abstract
    • Abstract
  • The study dealt with extraction of DNA with improved CTAB(cetyl-trimethyl-ammonium bromide) method from Ageratina adenophora Spreng.It showed that CTAB buffer supplemented with 2% PVP40(polyvinylpyrrolidone 40) and 0.4% β-mercaptoethanal was suitable for the genomic DNA extraction of A.adenophora,with OD260/OD280 value of 1.7-1.9,representing high purity of the extracted DNA and the complete elimination of protein,phenol,polyhexose and RNA.Thus the improved CTAB method was approved suitable for further AFLP analysis of A.adenophora.Some key factors affecting DNA extraction,restriction-ligase reaction,pre-amplification and amplification processes were studied and an optimized AFLP reaction system of A.adenophora was established.With this AFLP reaction system,clear DNA electrophoresis fingerprints of A.adenophora was obtained,and the reproducibility was high.
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