Genomic DNA Extraction Method and AFLP Amplification Reaction System of Ageratina adenophora Spreng.

The study dealt with extraction of DNA with improved CTAB(cetyl-trimethyl-ammonium bromide) method from Ageratina adenophora Spreng.It showed that CTAB buffer supplemented with 2% PVP40(polyvinylpyrrolidone 40) and 0.4% β-mercaptoethanal was suitable for the genomic DNA extraction of A.adenophora,with OD₂₆₀/OD₂₈₀ value of 1.7-1.9,representing high purity of the extracted DNA and the complete elimination of protein,phenol,polyhexose and RNA.Thus the improved CTAB method was approved suitable for further AFLP analysis of A.adenophora.Some key factors affecting DNA extraction,restriction-ligase reaction,pre-amplification and amplification processes were studied and an optimized AFLP reaction system of A.adenophora was established.With this AFLP reaction system,clear DNA electrophoresis fingerprints of A.adenophora was obtained,and the reproducibility was high.