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Tang Wei, Ouyang Fan, Guo Zhongchen. SOMATIC EMBRYOGENESIS FROM PROTOPLASTS OF LOBLOLLY PINE[J]. Plant Science Journal, 1998, 16(2): 106-110.
Citation: Tang Wei, Ouyang Fan, Guo Zhongchen. SOMATIC EMBRYOGENESIS FROM PROTOPLASTS OF LOBLOLLY PINE[J]. Plant Science Journal, 1998, 16(2): 106-110.
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SOMATIC EMBRYOGENESIS FROM PROTOPLASTS OF LOBLOLLY PINE

  • 1.

    State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, The Chinese Academy of Sciences Beijing 100080

  • 2.

    Institute of Botany, The Chinese Academy of Sciences Beijing 100093

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  • Received Date: June 01, 1997
  • Revised Date: July 17, 1997
  • Published Date: April 23, 1998
    Graphical Abstract
    • Abstract
  • Influences of basic media,protoplast density,and ABA concentration on somatic embryogenesis of loblolly pine were studied. The results show that DCR medium is in favor of the formation of somatic embryos from protoplasts. And the optimum protoplast density and ABA concentration for somatic embryogenesis are 7 ×104/mL and4 mg/L, respectively. The microscopical observation showed that embryonal suspensor masses developed firstly into early stage proembryos,then into late stage proembryos.These results lay the foundation of plantlet regeneration from protoplast of loblollypine.
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