Advance Search
Jiang Yueming, Giora Zauberman, Yoram Fuchs, Fu Jiarui. ANALYSIS OF LYCHEE POLYPHENOL OXIDASE ACTIVITY UNDER DIFFERENT CONDITIONS[J]. Plant Science Journal, 1998, 16(3): 207-212.
Citation: Jiang Yueming, Giora Zauberman, Yoram Fuchs, Fu Jiarui. ANALYSIS OF LYCHEE POLYPHENOL OXIDASE ACTIVITY UNDER DIFFERENT CONDITIONS[J]. Plant Science Journal, 1998, 16(3): 207-212.

ANALYSIS OF LYCHEE POLYPHENOL OXIDASE ACTIVITY UNDER DIFFERENT CONDITIONS

More Information
  • Received Date: April 07, 1997
  • Revised Date: September 21, 1997
  • Published Date: August 23, 1998
  • Lychee polyphenol oxidase was purified by ammonium sulfate fractionation,gel fiLtration and ion exchange chromatography. The enzyme was found to be homogenous by SDSP-AGE and shown to be activated by divalent canons such as Mn2+ and Ca2+ and long chainfatty acids,especially those with a high degree of unstaturation,and was markedly inhibitedby FeSO4 and SnCl2 and short chain fatty acids such as butyric acid using 4-methylcatechol assubstrate. Aslight decrease or increase was observed by added sodium dodecyl sulfate(SDS), hexadecyltri-methylammonium bromide (CATs) or triton X-100, respectively. Furthermore the effect of divalent cations on the activity of polyphenol oxidase was analysed.The optimum conditions for the activation process produced by the divalent canons had beenexamined with respect to canon concentration and pll of the reaction medium. This activationhad also been studied with regard to the kinetic parameters of the enzyme,which half-activation constants were in the order of 0. 8 mmol/L for Mn2+ and 1. 2 mmol/L for Ca2+. The Km decreased and the Vmax increased when Mn2+ and Ca2+ (5 mmol/L) were present.
  • Related Articles

    [1]Zhu Kai, Fan Yingxuan, Zuo Qihui, Tan Siyu, Liu Fenwu, Zhang Jian, Qin Junmei, Gao Lina. Photosynthetic limitation in soybean in response to soil water-nitrogen interactions and its relationship with leaf water use efficiency[J]. Plant Science Journal, 2024, 42(5): 634-643. DOI: 10.11913/PSJ.2095-0837.24036
    [2]LI Zhao-Bo, LI Wei, GU Shu-Ping, CAO Yu, YIN Li-Yan. Effects of Low CO2 on Photosynthetic Characteristics of Ottelia alismoides and Hydrilla verticillata[J]. Plant Science Journal, 2011, 29(2): 226-233.
    [3]QIU Zong-Bo, ZHU Feng-Rong, LI Jin-Ting, GUO Jun-Li. Effects of CO_2 Laser Pretreatment on Activities of Antioxidative Enzymes in Root of Wheat Seedlings under Water Stress[J]. Plant Science Journal, 2010, 28(1): 63-66.
    [4]QIU Zong-Bo, LI Fang-Min, WANG Fang, YUE Ming. Effects of CO2 Laser on Glutathione-dependent Antioxidative System in Wheat Seedling under Drought Stress[J]. Plant Science Journal, 2008, 26(4): 402-406.
    [5]LI Fang-Min, WANG Jun, CHEN YU-Ping, ZOU Zhi-Rong, WANG Xun-Ling, YUE Ming. The Combined Effects of Enhanced UV-B Radiation and Doubled CO2 Concentration on the Growth,Fruit Quality and Yield of Tomato in Winter Plastic Greenhouse[J]. Plant Science Journal, 2006, 24(1): 49-53.
    [6]XU Min, CHEN Shan, LIU Guo-Xiang, HU Zheng-Yu. Pilot Study of Physiological and Morphological Acclimation of Scenedesmus armatus Under Extreme-High-CO2-Stress[J]. Plant Science Journal, 2004, 22(5): 439-444.
    [7]LI Yun-Guang, HU Zheng-Yu. Studies on the Cultivation of Nostoc flagelliforme[J]. Plant Science Journal, 2003, 21(5): 411-414.
    [8]XIA Jian-Rong, GAO Kun-Shan. Change of Cell Structure of Chlorella pyrenoidosa Grown under Different CO2 Concentration[J]. Plant Science Journal, 2002, 20(5): 403-404.
    [9]SUN Gu-Chou, LIN Zhi-Fang, LIN Gui-Zhu. The Carboxylation Rate of Rubisco and Activity of Carbonic Anhydrase in Plants from a Subtropical Forest Grown at Different Light Intensity[J]. Plant Science Journal, 2001, 19(4): 304-310.

Catalog

    Article views (2260) PDF downloads (1351) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return