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Jiang Yueming, Giora Zauberman, Yoram Fuchs, Fu Jiarui. ANALYSIS OF LYCHEE POLYPHENOL OXIDASE ACTIVITY UNDER DIFFERENT CONDITIONS[J]. Plant Science Journal, 1998, 16(3): 207-212.
Citation: Jiang Yueming, Giora Zauberman, Yoram Fuchs, Fu Jiarui. ANALYSIS OF LYCHEE POLYPHENOL OXIDASE ACTIVITY UNDER DIFFERENT CONDITIONS[J]. Plant Science Journal, 1998, 16(3): 207-212.

ANALYSIS OF LYCHEE POLYPHENOL OXIDASE ACTIVITY UNDER DIFFERENT CONDITIONS

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  • Received Date: April 07, 1997
  • Revised Date: September 21, 1997
  • Published Date: August 23, 1998
  • Lychee polyphenol oxidase was purified by ammonium sulfate fractionation,gel fiLtration and ion exchange chromatography. The enzyme was found to be homogenous by SDSP-AGE and shown to be activated by divalent canons such as Mn2+ and Ca2+ and long chainfatty acids,especially those with a high degree of unstaturation,and was markedly inhibitedby FeSO4 and SnCl2 and short chain fatty acids such as butyric acid using 4-methylcatechol assubstrate. Aslight decrease or increase was observed by added sodium dodecyl sulfate(SDS), hexadecyltri-methylammonium bromide (CATs) or triton X-100, respectively. Furthermore the effect of divalent cations on the activity of polyphenol oxidase was analysed.The optimum conditions for the activation process produced by the divalent canons had beenexamined with respect to canon concentration and pll of the reaction medium. This activationhad also been studied with regard to the kinetic parameters of the enzyme,which half-activation constants were in the order of 0. 8 mmol/L for Mn2+ and 1. 2 mmol/L for Ca2+. The Km decreased and the Vmax increased when Mn2+ and Ca2+ (5 mmol/L) were present.
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