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Zhang Xiaoguo, Liu Yule, Yang Qingping, Tian Bo. CONSTRUCTION OF EXPRESSION VECTORS FOR CEREAL MALE STERILITY AND ITSFERTILITY RESTORATION[J]. Plant Science Journal, 2000, 18(1): 15-20.
Citation: Zhang Xiaoguo, Liu Yule, Yang Qingping, Tian Bo. CONSTRUCTION OF EXPRESSION VECTORS FOR CEREAL MALE STERILITY AND ITSFERTILITY RESTORATION[J]. Plant Science Journal, 2000, 18(1): 15-20.
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CONSTRUCTION OF EXPRESSION VECTORS FOR CEREAL MALE STERILITY AND ITSFERTILITY RESTORATION

  • 1.

    College of Life Sciences, Wuhan University Wuhan 430072

  • 2.

    Institute of Microbiology, The Chinese Academy of Sciences Beijing 100080

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  • Received Date: October 17, 1998
  • Revised Date: December 29, 1998
  • Published Date: January 23, 2000
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    • Abstract
  • The tapetum- promoter(CA55) was amplified from the total DNAof Zea mays L.by polymerase chain reaction, and was fused with barnase(BN) and barstar(BS) genes, respectively.The male sterile gene.CA55BNNOSand its fertility restoration gene CA55BSNOSwere construced.They were cloned into p CAMBIA3300, respectively.The expression vectors for cereal male sterility and its fertility restoration were obtained.
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