ISOLATION, PURIFICATION AND CHARACTERIZATION OF POLYSACCHARIDES FROM PRASIOLA YUNNANICA

The polysaccharides extracted from prasiola yunnanica.by hot water are EPS (extracellular polymeric substances).They are deproteinized by Sevag method for several times and purified by DEAE-cellulose and Sephadex G-200 chromatography column in turn.The crude polysaccharides were proved to have a little starch and some SO₄^2- by iodine indicator and BaCl₂ solution respectively.Agarose gel electrophoresis shows the existence of sugar and protein which are inhomoaenous.It has 13.54% of protein essayed by Lowry method, and 78% of sugar by benzene-H₂SO₄ method. And the rest are ash and water. The components of the crude polysaccharides were identified by paper chromatography and TLCwhich were D-Mannose, D-Ribose and L-Rhamnose with the ratio of 4: 15: 1. D-Ribose in the algal polysaccharides studies is quite rare.