Identification of Hybrid of Oryza sativa×O.meyeriana Using Genomic in situ Hybridization
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Graphical Abstract
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Abstract
With biotin-labelled total genomic DNAs from Chinese wild rice, Oryza meyeriana as probe and 4-fold unlabelled total genomic DNA from cultivated rice,O.sativa IR 36 as blocking,genomic in situ hybridization was applied to somatic chromosome preparations of O.sativa×O.meyeriana F1 hybrid.When detected with anti-biotin-FITC,O.meyeriana chromosomes gave gree-nish-yellow fluorescence while cultivated rice chromosomes fluoresced red with PIcounterstain.The area of signal on each wild chromosome was small.It suggested that a great part of DNA sequence in O.meyeriana is homologous to cultivated rice.
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