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HOU Xue-Wen, ZHENG Sui-Ping, GUO Yong. The Culturing Process and Kinetic Model Construction of Hibiscus sabdariffa Suspension Culture[J]. Plant Science Journal, 2001, 19(4): 317-322.
Citation: HOU Xue-Wen, ZHENG Sui-Ping, GUO Yong. The Culturing Process and Kinetic Model Construction of Hibiscus sabdariffa Suspension Culture[J]. Plant Science Journal, 2001, 19(4): 317-322.
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The Culturing Process and Kinetic Model Construction of Hibiscus sabdariffa Suspension Culture

  • 1.

    College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China

  • 2.

    Bioengineering Depa rtment, South China University of Technology, Guangzhou 510641, China

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  • Received Date: November 22, 2000
  • Revised Date: May 26, 2001
  • Published Date: July 23, 2001
    Graphical Abstract
    • Abstract
  • A tamed Roselle(Hibiscus sabdariffa L.) callus strain was cultured in Gamborg B5 liquid medium adding 3% sucrose,4.5μmol/L 2,4-D and 2.3μmol/L Kinetin(Kt). The growth and ubiquinone accumulation of Roselle cell, the change of pH, nutrient assimilation in the medium were studied. Anon structured kinetic model was constructed by using these data. Verification showed that the prediction of cell growth, total sugar assumption and ubiquinone accumulation by these model had a very high coincidence with the experimental results.
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