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Li Changgong, Zhou Chang, Yang Hongyuan, Li Xianghua, Zhang Qifa. STUDIES ON POLLEN-SOMATIC PROTOPLAST FUSION BETWEEN BRASSICA CHINENSIS AND B. JUNCEA[J]. Plant Science Journal, 1996, 14(4): 289-293.
Citation: Li Changgong, Zhou Chang, Yang Hongyuan, Li Xianghua, Zhang Qifa. STUDIES ON POLLEN-SOMATIC PROTOPLAST FUSION BETWEEN BRASSICA CHINENSIS AND B. JUNCEA[J]. Plant Science Journal, 1996, 14(4): 289-293.
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STUDIES ON POLLEN-SOMATIC PROTOPLAST FUSION BETWEEN BRASSICA CHINENSIS AND B. JUNCEA

  • 1.

    Laboratory of Plant Reproductive Biology, College of Life Science, Wuhan University Wuhan 430072

  • 2.

    The State Key Laboratory in Genetic Improvement of Crops Huazhong Agricultural University Wuhan 430070

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  • Received Date: June 29, 1995
  • Revised Date: August 14, 1995
  • Published Date: December 23, 1996
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    • Abstract
  • The fusion between young plooen (mid-late unicellular to early bicellular) protoplasts of Brassica chinensis and hypocotyl protoplasts of B. juncea was induced by polyethylene glycol(PEG). The results showed that among four methods tried the modified Terada et al's(1987)method was the best. PEG- treated protoplasts divided and formed calli.Afew calli differentiated into roots, but no shoots were regenerated. Arbitrarily selected 5 lines originated from PEG-treated protoplasts were analysed with random amplified polymorphic DNA(RAPD) markers, and one clone had a combination of both B. chinensis and B. juncea DNAband pattern. The conclusion is that this was a gameto-somatic hybrid clone resulted from the fusion between pollen protoplast of B.chinensis and hypocotyl protoplast of B. juncea.
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