CRYOPRESERVATION AND FORMATION OF REGENERATIVE PLANTLETS OF CALLUS FROM WILD RICE

The tissue culture and cryopreservation of 7 species of wild rice,were studied. Main results are as follows:1.By preculturing→pretreating→freezing →liquid nitrogen preserving→rapid thawing,the highest cell survival percentage of wild rice calli was 87. 9% 2.The best cryoprotectant wsa 10% DMSO plus 8% glucose for wild rice cryopreservation.And the optimal freezing procedure was:0℃(这里有图片19960309-247-1.GIF)-40℃,60min→liquid nitrogen.3.Regenerative plantlets were obtained after cryopreservation in Oryza rufipogon,O.latifolia and O. meyeriana. 4. Embryogenic callus formed after cryopreservation in O.meyeriana. And a large number of regenerative plantlets were obtained through the somatic embryogenesis way.