Effects of Medium Concentration on Callus Growth and Secondary Metabolites of Eucommia ulmoides

The effects of medium concentration on callus growth and the accumulation of chlorogenic acid and flavonoids of Eucommia ulmoides were investigated. The treatments were designed in B₅ medium supplemented with 0.5 mg/L NAA + 0.5 mg/L BA.The results showed that the callus growth was increased when KNO₃ content was increased,whereas chlorogenic acid and flavonoids contents were highest when KNO₃ concentration was 2/3 of original amount in B₅ medium.The 4/3 content of (NH₄)₂SO₄ in B₅ medium were best for the callus growth and flavonoids content and yield, whereas chlorogenic acid content was highest when (NH₄)₂SO₄ content was 1/3 of original amount in B₅ medium.The callus growth was highest when MgSO₄ concentration was 2/3,whereas chlorogenic acid and flavonoids contents were highest when MgSO₄ content was 1/3 in B₅ medium. The original content of NaH₂PO₄,CaCl₂ and MnSO₄ in B₅ medium were best for the callus growth and the accumulation of chlorogenic acid and flavonoids. The contents of ZnSO₄ and FeSO₄ in B₅ medium was best for the callus growth but chlorogenic acid and flavonoids contents was highest when ZnSO₄ and FeSO₄ concentration was 1/3 in B₅ medium.