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WEI Shan-Jun, SUN Zhen-Yuan, QIAN Yong-Qiang, YU Long-Jiang, Han Lei. Plant Regeneration from Mature Embryo of Zoysia sinica Hance in vitro[J]. Plant Science Journal, 2004, 22(5): 455-458.
Citation: WEI Shan-Jun, SUN Zhen-Yuan, QIAN Yong-Qiang, YU Long-Jiang, Han Lei. Plant Regeneration from Mature Embryo of Zoysia sinica Hance in vitro[J]. Plant Science Journal, 2004, 22(5): 455-458.
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Plant Regeneration from Mature Embryo of Zoysia sinica Hance in vitro

  • 1.

    College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan430074, China

  • 2.

    Research Institute of Forestry, Chinese Academy of forestry, Beijing100091, China

  • 3.

    Beijing Forestry University, Beijing 100083, China

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  • Received Date: November 26, 2003
  • Revised Date: February 25, 2004
  • Published Date: October 27, 2004
    Graphical Abstract
    • Abstract
  • Mature seeds were used as explant to study the callus induction, subculture, and plant regeneration of Zoysia sinica Hance. The results showed that the maximum rate of callus induction was 43.0% and occurred on the modified MS medium (MSm) supplemented with 2.5 mg/L 2,4-D, 0.25 mg/L 6-BA, and 1-2 mg/L thiamine. The optimum subculture medium was MSm containing 0.1 mg/L 6-BA and 2.0 mg/L 2,4-D. 30%-60% calli type of white to yellowish could regenerate plants on MS medium free of growth regulators.
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