Isolation,Purification of Glycoproteins from Seed of Nelumbo nucifera Gaertn.Fujian and its Effect on Scavenging Free Radicals

A bioactive glycoprotein(GLP) was isolated and purified from seed of Nelumbo nucifera Gaertn.Fujian and it plays a role in scavenging free radicals.The glycoprotein was precipitated in 60% saturanted solution of (NH₄)₂SO₄ first,was purified by Con-A Sepharose 4B affinity chromatography and by Sephadex G-150 gel filtration chromatsgraphy consecutively, and last was aftained two parts of glycoproteins: GLP-Ⅰ of molecular weight is 25 kD and GLP-Ⅱ of molecular weight is 92.5 kD.A GLP Ⅱ is primary part in GLP.Contents of protein and total sugars of GLP are 58.8% and 36.4% respectively.By TLC and GC analysis resulted that, glycosyls of GLP-Ⅰ are L-rhamnose, D-arabinose, D-mannose and D-glucose, and its molar ratios of neutral sugars are 1.08:0.92:2.54:4.15 and glycosyls of GLP-Ⅱ are L-rhamnose, D-arabinose,D-manmose, L-xylose,D galactose,D-glucose and D-glucuronic acid,and its molar ratio of neutral sugars are 1.25:1.03:2.85:0.94:3.16:4.65.Phamarcological tests showed that, GLP-Ⅰ and GLP-Ⅱboth could scavenigered free radical and scavenging effect of GLP-Ⅱis higher than of GLP-Ⅰ.