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SHAO Jin-Zhen, QIU Chang-En, DING Yi. Comparison of the Effect of Different Extractants on Hordein and Gliadin Extraction by A-PAGE[J]. Plant Science Journal, 2003, 21(3): 262-266.
Citation: SHAO Jin-Zhen, QIU Chang-En, DING Yi. Comparison of the Effect of Different Extractants on Hordein and Gliadin Extraction by A-PAGE[J]. Plant Science Journal, 2003, 21(3): 262-266.
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Comparison of the Effect of Different Extractants on Hordein and Gliadin Extraction by A-PAGE

  • 1.

    Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan 430072, China

  • 2.

    Department of Biology, Hubei Normal College, Huangshi 435002, China

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  • Received Date: October 14, 2002
  • Revised Date: May 10, 2003
  • Published Date: June 23, 2003
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    • Abstract
  • The alcohol-soluble proteins (hordeins from barley, gliadins from wheat) were extracted from the embryo-removed seeds of four barley cultivars (Hordeum vulgare L.) and three wheat cultivars (Triticum aestivum L.) with different extracting solvents. The results revealed that the extraction of hordeins and gliadins was most efficient with extraction solution composed of the 2-chloroethanol (25%) and 2-mercaptothanol (1%). Using this extractant, more hordein or gliadin protein bands could be resolved by polyacrylamide gel electrophoresis under acid pH condition.
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