Study on the Editing Sites in Transcripts of Functional Genes of HL-cytoplasmic Male Sterility Rice Mitochondria during Microgametogenesis

RNA editing exists extensively in the higher plant mitochondria,and is a required step for forming functional proteins.The editing of mitochondrial gene atp6,coxⅡ and orf H79 was studied with(using) the tetrad anthers,uninucleate and dinucleate pollens of gametophytice male sterility line YueTai A,maintainer line YueTai B,and the F₁ hybrid HongLian You6 of HL-type cytoplasmic male sterility(CMS) rice.The results indicated the editing capability of atp6 gene was influenced markedly by nuclear restorer gene.The atp6 transcript was not edited or partially edited in male sterility line,but the higher editing capability was found in the F₁ hybrid containing restorer gene.There was no difference in the editing level of coxⅡ gene among three lines,and the chimeric gene,orf H79 was not edited in all materials.So it is guessed that there is a close association between HL-CS and the loss of editing capability of atp6 gene.