Advance Search
Yang Xiong, Zhai Yihua, Zou Guolin. PURIFICATION AND PROPERTIES OF MANGANESE SUPEROXIDE DISMUTASE IN LEEK MITOCHONDRIA[J]. Plant Science Journal, 1998, 16(4): 305-309.
Citation: Yang Xiong, Zhai Yihua, Zou Guolin. PURIFICATION AND PROPERTIES OF MANGANESE SUPEROXIDE DISMUTASE IN LEEK MITOCHONDRIA[J]. Plant Science Journal, 1998, 16(4): 305-309.

PURIFICATION AND PROPERTIES OF MANGANESE SUPEROXIDE DISMUTASE IN LEEK MITOCHONDRIA

More Information
  • Received Date: July 21, 1997
  • Revised Date: November 20, 1997
  • Published Date: December 23, 1998
  • Asuperoxide dismutase from leek mitochondria has been purified to homogeneith by ammonium sulfate fraction, DEAE-Sephacel chromotography and Sephadex G-200 gel filtration.2.5 mg purified enzyme was obtained from 6000 g leaves.The specific activity of the purified enzyme is 1200 U/mg protein.The enzyme is not sensitive to inhibitors:KCN nad H2O2.It has good heat .stability.The largest amount of ultraviolet absorption is at 280 urn.The molecular weight of the enzyme is about 82000 Das assayed by Sephadex G-200 gel filtration,that of the enzyme subunit is about 22000 Das tested by SDS-polyacrylamide gel electrophoresis.The N-terminal amine acid of the enzyme is valine as tested by DNS-CI.These results show the enzyme is a manganese superoxide.
  • Related Articles

    [1]Liu Yan-Li, Jin Xiao-Fang, Ma Lin-Long, Cao Dan, Gong Zi-Ming, Wei Chao-Ling. Isolation and purification of mesophyll protoplasts from the leaves of Camellia sinensis[J]. Plant Science Journal, 2017, 35(6): 908-911. DOI: 10.11913/PSJ.2095-0837.2017.60908
    [2]ZHANG Hong, CHEN Zu-Yu, LI Yang-Sheng, ZHU Ying-Guo. The Mitochondria Genomic Mutation and Function in the Cytoplasmic Male Sterile Line of Maxie Rice[J]. Plant Science Journal, 2007, 25(3): 313-315.
    [3]KONG Jin, TAN Yan-Ping, CHEN Zu-Yu, LI Shao-Qing, ZHU Ying-Guo. Study on the Editing Sites in Transcripts of Functional Genes of HL-cytoplasmic Male Sterility Rice Mitochondria during Microgametogenesis[J]. Plant Science Journal, 2006, 24(2): 95-99.
    [4]GAO Ju Yi, CHEN Yan. Isolation,Purification of Glycoproteins from Seed of Nelumbo nucifera Gaertn.Fujian and its Effect on Scavenging Free Radicals[J]. Plant Science Journal, 2003, 21(2): 175-178.
    [5]WANG Li, YI Ping, WAN Cui-Xiang, ZHU Ying-Guo. AP-PCR Analysis for Mitochondria DNA of CMS Rice Honglian Type[J]. Plant Science Journal, 2002, 20(6): 405-408.
    [6]Ma Changyan, Wei Jincheng, Cheng Guangyu, Wu Guorong. PURIFICATION AND PROPERTIES OF AN IRO CONTAINING SUPEROXIDE DISMUTASE FROM SPINACH LEAVES,SPINACEA OLERACEA[J]. Plant Science Journal, 1999, 17(2): 122-126.
    [7]Zhai Yihua, Zou Guolin, Yang Xiong. PURIFICATION AND PROPERTIES OF SUPEROXIDE DISMUTASE IN LEEK PLASMA[J]. Plant Science Journal, 1998, 16(1): 18-22.
    [8]Gong Xiaosong, Zeng Fuhua, Yan Longfei. AN EFFICENT METHOD FOR THE PURIFICATION OF CHLOROPLAST DNA FROM HIGHER PLANTS[J]. Plant Science Journal, 1994, 12(3): 280-280.
    [9]Chen Fan, Chen Haifang, Fu Jun. PURIFICATION AND PHYSICHEMICAL CHARACTERIZATION OF A LECTIN FROM WISTARIA SINENSIS SWEET[J]. Plant Science Journal, 1994, 12(3): 270-276.
    [10]Cui Yunxia, Liu Shiye, Wang Guoliang, Zou Guolin, Guo Yunhua. PURIFICATION AND SOME PROPERTIES OF COPPER/ZINC SUPEROXIDE DISMUTASE IN ACTINIDIA CHINENSIS FRUITS[J]. Plant Science Journal, 1993, 11(1): 73-82.

Catalog

    Article views (1992) PDF downloads (2150) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return