Studies on Rapid Propagation Technology of Common Buckwheat Stem Segments

The effects of different hormone on axinary buds induction,tufty bud differentiation,and rooting were studied by means of orthogonal design and common buckwheat(Fagopyrum esculentum Moench)stem segment explants with a knot. The results showed that 6-BA is a major factor for induction of axianry buds and roots,and that TDZ is the key to induction of tufty bud.The optimal medium for inducing axillary bud development is MS₀+6-BA 1.0 mg/L+NAA 0.1 mg/L,with the rate of axillary bud development of 67.9%.Among them,the second and third knot can produce the rate of 80% or more.The MS₀ medium containing 6-BA 4.0 mg/L+TDZ 0.06 mg/L is better for inducing tufty buds with the differentiation rate of 166.7%.The optimum medium of root formation is 1/2 MS₀+6-BA 1.0 mg/L+NAA 1.0 mg/L,with the rooting rate of 82.8%.At last,a rapid propagation technology was developed for common buckwheat plants.