Prokaryotic Expression of DREB1A Transcription Factor

DREB1A is a member of the DREB transcription factors.Each DREB protein contains 58 conserved amino acids named as EREBP/AP2 domain,which binds cis-acting element named DRE(for dehydration-responsive element).A DREB transcription factor could regulate multiple functional genes related to dehydration,low temperature and high salt tolerance of plant.We cloned the DREB1A gene from Arabidopsis thaliana(Columbia ecotype) genome by PCR and constructed the prokaryotic express vector,which was transferred into E.coli BL21(DE3).Expression of the DREB1A protein was induced by 1 mmol/L IPTG,but the target protein obtained in the form of inclusion body.This work provides a foundation for further understanding of the interaction mechanism between DREB transcription factors and DRE cis-acting elements.