Factors of Flower Inducing and Histological Observation in Dioscorea zingberensis C.H.Wright in vitro

In vitro flower inducing system was established directly in tissue culture of inflorescences in Dioscorea zingberensis C.H.Wright.Study was made on the factors influencing the inducing efficiency.Inflorescences of D.zingberensis from five populations were used as explants and incubated in different mediums.New inflorescences were induced from the five populations successfully in various frequencies.Explants related to flower organs were capable to regenerate new inflorescences.Cytokinin was found to be the key factor for inducing these inflorescences.Among two kinds of cytolinin,benzyladenine(BA) was essential to induce inflorescences,while addition of kinetin(KT) and high concentration of Fe2+-EDTA would led to generation of vegetative buds.MS medium supplemented with 2.0 mg/L benzyladenine and 0.2 mg/L naphthaleneacetic acid(NAA) was optimal to inducing of inflorescences and following growth.Histological observation indicated the inflorescences regenerated from bracts and epidermal cell of the periant of the explants.