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LI Jing-Fang, HUANG Shao-Yi, TIAN Ren-Peng, ZHANG Luo-Zhen, FANG Cheng-Xiang. An Improved Method Suited to Extract Total RNA Used to RT-PCR from Fir Plant[J]. Plant Science Journal, 2004, 22(6): 551-556.
Citation: LI Jing-Fang, HUANG Shao-Yi, TIAN Ren-Peng, ZHANG Luo-Zhen, FANG Cheng-Xiang. An Improved Method Suited to Extract Total RNA Used to RT-PCR from Fir Plant[J]. Plant Science Journal, 2004, 22(6): 551-556.
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An Improved Method Suited to Extract Total RNA Used to RT-PCR from Fir Plant

  • College of Life Sciences, Wuhan University, Wuhan 430072, China

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  • Received Date: January 18, 2004
  • Revised Date: May 08, 2004
  • Published Date: December 25, 2004
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    • Abstract
  • Fir plants contain plenty of polysacchrides and polyphenols.Comparing several standard methods for extracting total RNA from fir plant, a modified method which can eliminate the interferences of polysacchrides and polyphenols in the plant tissues was constructed in the present study. The high-quality RNA sample obtained by the improved CTAB method used to RT-PCR (reverse transcriptase-polymerase chain reaction) of P450 gene study,and the result indicated that the method is easier for operation and is at lower cost.
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