Analysis of Total Proteins in Pollen of Humulus scandens Lour. in Wuhan Region of China by Two-dimensional Electrophoresis

The total proteins in pollen of Humulus scandens Lour.,one of the most popular aeroallergen in China,were analyzed by two-dimensional electrophoresis in current study.The total proteins of the pollen were extracted by trichloracetic acid(TCA) method,and then separated by isoelectric focusing as first dimension and SDS-PAGE as the second dimension.The spots of proteins were visualized by staining with Coomassie Brilliant Blue.After analysis with software(ImageMaster 2D),122 different proteins were detected;their isoelectric point(pI),Molecular weight(MW) and relative volume of each protein in the pollen were also discovered.This is the first high-resolution,two-dimensional protein map of the pollen of H.scandens Lour.in China.Our finding has built a solid foundation for identification,characterization,gene cloning and standardization of allergenic proteins in the pollen of H.scandens Lour.in further.