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QIU Zong-Bo, LI Fang-Min, WANG Fang, YUE Ming. Effects of CO2 Laser on Glutathione-dependent Antioxidative System in Wheat Seedling under Drought Stress[J]. Plant Science Journal, 2008, 26(4): 402-406.
Citation: QIU Zong-Bo, LI Fang-Min, WANG Fang, YUE Ming. Effects of CO2 Laser on Glutathione-dependent Antioxidative System in Wheat Seedling under Drought Stress[J]. Plant Science Journal, 2008, 26(4): 402-406.
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Effects of CO2 Laser on Glutathione-dependent Antioxidative System in Wheat Seedling under Drought Stress

  • 1.

    Key Laboratory of Resource Biology and Biotechnology in Western China(Northwest University), Ministry of Education, Xi’an 710069, China

  • 2.

    College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China

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  • Received Date: November 04, 2007
  • Revised Date: February 18, 2008
  • Published Date: August 28, 2008
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    • Abstract
  • The objective of this study was to determine the effects of CO2 laser(wavelength 10600 nm,power density 20.1 mW/mm2) on glutathione dependent antioxidative system in wheat seedling under drought stress.Germinating seeds were exposed to CO2 laser radiation for 0 min,1 min,3 min and 5 min,respectively,then they were treated with 10%(W/V) PEG6000 solution for 8 days.When the seedlings were 12-day-old,changes in the concentration of oxidized glutathione(GSSG),reduced glutathione(GSH),ascorbate(AsA) and the activities of glutathione reductase(GR),glutathione-S-transferase(GST),ascorbate peroxidase(APX) were measured to determine the effects of laser.The results showed that suitable laser treatment of seeds improved drought tolerance in wheat seedlings by decreasing the concentration of GSSG and increasing the activities of APX,GR,GST,AsA and GSH concentration.The results suggested that those changes in glutathione dependent antioxidative system were responsible for protection wheat against drought induced oxidative damage.
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