SUBUNITS OF PHYCOCYANIN AND F₆₇₈ PIGMENT-PROTEIN IN ANABAENA CYLINDRICA

A procedure has been derived for the purification of phycocyanin and its suhunits. Phycocyanin has the 360 nm peak and the 615 nm peak in absorption spectrum and two subunits were observed by sodium dodecyl-sulfate as solubilized agent in 100℃ 5 min. along with a procedure electrophoresis system. β subunit dominating the 578 nm peak in absorption spectrum and the 600nm peak in fluorescence spectrum was found. MW was 19.80±0.40 KD. In α subunit the 578 nm peak and 630nm peak became the dominant bands in absorption spectrum and MW was 17.35±0.38 KD.At liquid nitrogen temperature a main peak with 650nm and a peak with 635 nm were found in allophycocyanin.When phycobilisome-conatining vesicles suspended in higher salt concentration medium with 0.5M sucrose /0.5M phosphatecitrate /1.1% albumin were suspended in lower salt concentration medium with 0.5M sucrose/0.05M phosphate-citrate /1.1%albumin, a fluorescence emission with 678 nm was found (excited by light at 600 nm in liquid temperature). It was likely to he allophycocyanin B in Anabaena cylindrica.