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GAN Chun-Hui, RAN Yong, ZHAO Kai-Hong. Study on the Auto-assembly of Truncated Phytochrome from Synechocystis sp.PCC7120[J]. Plant Science Journal, 2005, 23(6): 505-510.
Citation: GAN Chun-Hui, RAN Yong, ZHAO Kai-Hong. Study on the Auto-assembly of Truncated Phytochrome from Synechocystis sp.PCC7120[J]. Plant Science Journal, 2005, 23(6): 505-510.
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Study on the Auto-assembly of Truncated Phytochrome from Synechocystis sp.PCC7120

  • College of Environmental Science & Engineering, Huazhong University of Science and Technology, Wuhan 430074, China

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  • Received Date: April 13, 2005
  • Revised Date: June 18, 2005
  • Published Date: December 27, 2005
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    • Abstract
  • Five truncated fragments of aphA gene were amplified from the genomic DNA of Synechocystis sp.PCC7120 by PCR,including aphA(26-320),aphA(27-320),aphA(28-320),aphA(29-320)and aphA(32-320).After expression via the vector pET30,reconstitutions of the expressed mutated apo-proteins with phycocyanobilin(PCB) were studied in vitro under certain conditions.The results showed:The chromoprotein that obtained from the reconstitution of AphA(26-320) had similar photochemical properties of plant phytochrome.The covalent binding of PCB with the chromoprotein was verified further by acid-urea denaturation and UV-induced fluorescence by Zn2+-SDS gel tests.The maximal Pr/Pfr absorption of AphA(26-320) was at 660/610 nm.The other four truncated fragments,AphA(27-320),AphA(28-320),AphA(29-320),AphA(32-320) could not auto-assembly with PCB in the similar conditions,as no reversible photochemistry was detected.The results implicates that the lyase domain of AphA existing in AphA(26-320).
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