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Xiao Zunan, Shen Dexu, Lin Bonian. CULTURE AND PLANT REGENERATION OF ACTINIDIA DELICIOSA PROTOPLASTS ISOLATED FROM COTYLEDON CALLUS[J]. Plant Science Journal, 1993, 11(3): 247-252.
Citation: Xiao Zunan, Shen Dexu, Lin Bonian. CULTURE AND PLANT REGENERATION OF ACTINIDIA DELICIOSA PROTOPLASTS ISOLATED FROM COTYLEDON CALLUS[J]. Plant Science Journal, 1993, 11(3): 247-252.
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CULTURE AND PLANT REGENERATION OF ACTINIDIA DELICIOSA PROTOPLASTS ISOLATED FROM COTYLEDON CALLUS

  • Department of Horticulture, Zhejiang Agricultural University, Hangzhou 310029

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  • Received Date: September 15, 1991
  • Revised Date: December 20, 1992
  • Published Date: September 23, 1993
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  • Callus lines of A11B2 and A16N1 were selected from cotyledon cultures on B5 and NN-69 media containing 1mg/L 2,4-D, respectively. When protoplasts isolated from callus line A11B2 were cultured on the modified B5 medium, cell division was arrested at the stage of cell clusters. However, when protoplasts isolated from callus line A16N1 were cultured on the modified NN-69 medium, they had divided to form cell groups, then developed into callus, from which plants were regenerated by the method of the stepwise induction of organgenesis.
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