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Shi Shuwen, Meng Jinling. STUDIES ON IN VITRO CULTURE OF POLLINATED OVARY IN INTERSPECIFIC CROSSES BETWEEN BRASSICA SPECIES[J]. Plant Science Journal, 1995, 13(1): 8-14.
Citation: Shi Shuwen, Meng Jinling. STUDIES ON IN VITRO CULTURE OF POLLINATED OVARY IN INTERSPECIFIC CROSSES BETWEEN BRASSICA SPECIES[J]. Plant Science Journal, 1995, 13(1): 8-14.
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STUDIES ON IN VITRO CULTURE OF POLLINATED OVARY IN INTERSPECIFIC CROSSES BETWEEN BRASSICA SPECIES

  • Department of Agronomy, Huazhong Agricultural University Wuhan 430070

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  • Received Date: August 10, 1993
  • Revised Date: November 01, 1993
  • Published Date: January 23, 1995
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  • The present paper reports on the method of in vitro culture of pollinated ovary in interspecific crosses, Brassica carinata×B.campestris,B.carinata×B.napus and B.carinata×B.juncea.Experimental result indicated that optimum time in vitro of ovaries of 3 kinds of interspecific crosses was 5-10、7-12 and 10-17 days after pollination, respectively.Through ovary culture, hybrid seedlings were obtainted in10 crosses.Casein hydrolysate was of no advantage to the development of embryos.0.2mg/L BA added to MS media promoted embryo formation.MS and L.S.M.medium as well as the culture technique used in this experiment was more fit to culture pollinated ovaries in B.carinata×B.campestris and B.carinata×B.napus.
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