CYTOGENETIC STUDIES ON REGENERATED PLANTS DERIVED FROM PROTOPLASTS OF ACTINIDIA DELICIOSA Ⅰ.VARIATION OF CHROMOSOME NUMBER OF SOMATIC CELLS

Chromosome number of stem tip cells of the pistillate plant,Actinidia deliciosa line No.26 and that of regenerated plants derived from protoplasts,which were isolated from leaf calli of the pistillate plant,were studied.It was indicated that the chromosome number of the pistillate plant was 2n=6x=174,and that of the 29 tested regenerated plant was variable obviously.Aneuploid occupied 72.4% of total regenerated plants tested.The chromosome number of most of the regenerated plants was between 142-310.Among them,plants with chromosome number of 2n=6x=174 were 20.7%,that of less than 174 were 31.0% and that of more than 174 were 48.3%.Some abnormal phenomena,such as chromosome bridges,fragments and lagging chromosomes appeared at anaphase of stem tip cells in a few plants.It was discussed about the phenomena mentioned above.