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Li Xuebao, Bai Yongyan, Wei Zhizning, Xu Zhihong. FACTORS INFLUENCIMG GENETIC TRANSFORMATION OF HYPOCOTYL PROTOPLASTS OF PHASEOLUS AUREUS (MUNG BEAN)[J]. Plant Science Journal, 1994, 12(1): 37-43.
Citation: Li Xuebao, Bai Yongyan, Wei Zhizning, Xu Zhihong. FACTORS INFLUENCIMG GENETIC TRANSFORMATION OF HYPOCOTYL PROTOPLASTS OF PHASEOLUS AUREUS (MUNG BEAN)[J]. Plant Science Journal, 1994, 12(1): 37-43.

FACTORS INFLUENCIMG GENETIC TRANSFORMATION OF HYPOCOTYL PROTOPLASTS OF PHASEOLUS AUREUS (MUNG BEAN)

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  • Received Date: June 12, 1992
  • Revised Date: November 25, 1992
  • Published Date: January 23, 1994
  • In this paper,we studied on factors influencing transformation of hypocotyl protoplasts of mung bean (Phaseolus aureus).The experimental result showed th t lower pH,phosphate starvation and acetosyringone could activate the expression of the vir genes of Ti-plasmid in Agrobacterium tumefaciens and promote T-DNA transfer into mung bean cells to some extent through transient expression of GUS activity in mung bean cells. Higher pH and concentration of Ca2+ promoted transient expression of GUS gene in PEG-mediated DNA direct transfer.The activity of GUS gene increased with the increase of foreign plasmid DNA concentration in the range of 10-100μg. The transformed calli from mung bean hypocotyl protoplasts were selected on MSO medium containing 50 mg/L chloramphanicol after the protoplasts were cocultivated with A.tumefaciens strain C58C1 (pBZ6111 ),and confirmed by analysis of nopaline synthetase (NOS) activity.
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