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JIA Li-Li, ZHOU Ming, SU Ping, ZHAO Kai-Hong. In vivo Reconstitution of Anabaena sp.PCC7120 Core-membrane Linker Protein ApcE with Phycocyanobilin[J]. Plant Science Journal, 2006, 24(4): 287-291.
Citation: JIA Li-Li, ZHOU Ming, SU Ping, ZHAO Kai-Hong. In vivo Reconstitution of Anabaena sp.PCC7120 Core-membrane Linker Protein ApcE with Phycocyanobilin[J]. Plant Science Journal, 2006, 24(4): 287-291.

In vivo Reconstitution of Anabaena sp.PCC7120 Core-membrane Linker Protein ApcE with Phycocyanobilin

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  • Received Date: January 15, 2006
  • Revised Date: March 29, 2006
  • Published Date: August 25, 2006
  • The deletion mutated core-membrane linker protein ApcE(1-240) from Anabaena sp.PCC7120 were overexpressed in E.coli.The activities of expressed proteins were studied through in vivo reconstitution tests,where phycocyanobilin-biosynthesizing gene(ho1 and pcyA) were transformed into E.coli.Through the absorption and the fluorescence spectra,the results show ApcE(1-240) could bind covalently phycocyanobilin(PCB) in an autocatalytic reaction.The presence of 4 mol/L urea is necessary to solubilize the resulting biliprotein,and it has absorption maxima(λmax=660 nm) and fluorescence maxima(λmax=668 nm).
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