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Ke Xiayi, Huang Yue, Shi Heping, Li Baojian. A STUDY ON GENE TRANSFORMATION OF IMMATURE WHEAT EMBRYOS BY ELECTROPORATION[J]. Plant Science Journal, 1997, 15(2): 103-107.
Citation: Ke Xiayi, Huang Yue, Shi Heping, Li Baojian. A STUDY ON GENE TRANSFORMATION OF IMMATURE WHEAT EMBRYOS BY ELECTROPORATION[J]. Plant Science Journal, 1997, 15(2): 103-107.
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A STUDY ON GENE TRANSFORMATION OF IMMATURE WHEAT EMBRYOS BY ELECTROPORATION

  • Biotechnology Research Center, Zhongshan University Guangzhou 510275

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  • Received Date: December 15, 1995
  • Revised Date: April 28, 1996
  • Published Date: April 23, 1997
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    • Abstract
  • By use of the HPES-3 pulse discharging electroporation system that is developed by our laboratory, we have transferred the bar gene(phosphinothricin acetyltransferase gene) in plasmid pDM302 into the immature embryos of a spring wheat cultivar (Triticum aesivum L.) "T2003".After selection for 2～3 months, we have obtained the ppt (phosphinothricin)-resistant callus, from which regenerated a wheat plantlet.PCR analysis and DNA dot hybridization showed the foreign bar gene had been integrated into the genomes of the transformed wheat plantlet.
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